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Method for removing interference to tba detection, tc kit and tg kit

A kit and reagent technology, applied in TC kits and TG kits, can eliminate the interference to TBA detection, and can solve the problems of TBA reagent measurement interference, cross-contamination, wrong TBA measurement results, etc.

Active Publication Date: 2021-09-14
WUHAN LIFE ORIGIN BIOTECH LTD
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Problems solved by technology

[0002] Total cholesterol (COD-CE-PAP method) assay kit (TC kit) and triglyceride (GPO-PAP method) assay kit (TG kit) In order to ensure the accuracy of the measured value of the reagent, gallbladder is added to the reagent Sodium cholate, but when detected on the biochemical analyzer, the sodium cholate in the reagent will interfere with the measured value of the TBA reagent due to the unclean cleaning of the reaction cup or the sampling needle of the biochemical analyzer, which may lead to errors in the TBA measured value
When multiple reagents of biochemical instruments are detected, cross-contamination is a relatively complicated and difficult problem to solve. The total cholesterol (COD-CE-PAP method) (TC kit) and triglyceride (GPO-PAP method) provided by the present invention The determination kit (TG kit) can eliminate the interference caused by the simultaneous detection with the TBA reagent; at the same time, it can also solve the obvious problem of most reagents on the market because they contain pungent-smelling phenolic substrates after the reagent is opened. Problems with unpleasant, pungent odors

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  • Method for removing interference to tba detection, tc kit and tg kit

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[0013] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0014] When the TBA kit is tested, if the kit tested on the same biochemical analyzer at the same time contains sodium cholate, the cuvette or the sampling needle of the biochemical analyzer may not be cleaned cleanly, which may interfere with the measured value of the TBA reagent.

[0015] In order to eliminate the interference of sodium cholate on TBA detection, it is necessary to remove the sodium cholate in the interfering kit, but only removing sodium cholate will cause the accuracy of the interfering kit itself to deteriorate and fail to achieve its its own testing purpose. Therefore, in the...

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Abstract

The invention relates to the technical field of kits, and specifically discloses a method for removing interference to TBA detection, wherein sodium cholate is removed from a kit that interferes with TBA detection, and phenolic substrates are replaced with aniline derivatives; and Surfactants are added to kits that interfere with TBA detection. Under the premise that the accuracy of the kit can be ensured, the method can also eliminate its interference on TBA detection, reduce the irritation of the reagent, and reduce the harm to the human body.

Description

technical field [0001] The invention relates to the technical field of kits, in particular to a method for removing interference to TBA detection, a TC kit and a TG kit. Background technique [0002] Total cholesterol (COD-CE-PAP method) assay kit (TC kit) and triglyceride (GPO-PAP method) assay kit (TG kit) In order to ensure the accuracy of the measured value of the reagent, gallbladder is added to the reagent Sodium cholate, but when tested on the biochemical analyzer, the sodium cholate in the reagent will interfere with the measured value of the TBA reagent due to the unclean cleaning of the cuvette or the sampling needle of the biochemical analyzer, which may lead to errors in the TBA measured value. When multiple reagents of biochemical instruments are detected, cross-contamination is a relatively complicated and difficult problem to solve. The total cholesterol (COD-CE-PAP method) (TC kit) and triglyceride (GPO-PAP method) provided by the present invention The deter...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/61C12Q1/60C12Q1/48C12Q1/44C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/44C12Q1/485C12Q1/60C12Q1/61C12Q2326/96G01N2333/904G01N2333/91215G01N2333/92
Inventor 龚婷赵畅吴年芬凡速朋梁艳舒芹张雪娇赵愿安
Owner WUHAN LIFE ORIGIN BIOTECH LTD