Rice seedling culture substrate and preparation method thereof, and organic acid soil and preparation method thereof
A technology for raising seedling substrate and rice, applied in the agricultural field, can solve the problems of transportation, storage, inconvenient use, environmental pollution, unsafe operation, etc., and achieve the effects of improving the utilization rate of biotransformation, shortening the fermentation period, and improving soil vitality.
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Embodiment 1
[0050] The preparation method of organic acid soil is as follows:
[0051] 1) Cultivation of Trichoderma and Aspergillus niger: Inoculate Trichoderma and Aspergillus niger into PDA medium respectively, after culturing at 28°C for 3-5 days, collect spores; inoculate spores on wheat grains, culture at 28°C for 7 days, then dry naturally in the shade Finally, crush the wheat grains for later use;
[0052] 2) Cultivation of acetic acid bacteria: 10 g of yeast extract, 10 g of glucose, 1 L of constant volume, sterilized at 121°C for 20 minutes, then added 1% absolute ethanol, 1% acetic acid, 150 rpm / min after inoculation of acetic acid bacteria, and cultured at 30°C for 24 hours;
[0053] 3) Yeast culture: 10 g of yeast extract, 10 g of peptone, 20 g of glucose, constant volume of 1 L, sterilized at 121 °C for 20 min, 150 rpm / min after yeast inoculation, and cultured at 28 °C for 24 h;
[0054] 4) Cultivation of Bacillus subtilis: 3 g of yeast powder, 5 g of sodium carboxymethyl c...
Embodiment 2
[0061] The specific operation steps are consistent with Example 1, except that the volume ratio of Trichoderma, Aspergillus niger, Bacillus subtilis, yeast and acetic acid bacteria is 1:1.5:0.5:1.5:0.5.
Embodiment 3
[0063] The specific operation steps are consistent with Example 1, except that the volume ratio of Trichoderma, Aspergillus niger, Bacillus subtilis, yeast and acetic acid bacteria is 1:2.5:1:2.5:1.
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