Gene chip hybridization box, gene chip set and gene chip hybridization method
A gene chip hybridization and gene chip technology, which is applied in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the problems of hybridization liquid not being covered, chip surface contact hybridization, etc.
Pending Publication Date: 2020-10-27
生捷科技(杭州)有限公司 +1
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Problems solved by technology
[0006] However, when reducing the amount of hybridization solution, there is a risk that the hybridization solution does not cover the surface of the chip, so that it cannot fully contact and hybridize with the chip surface
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[0048] In this application, unless otherwise stated, the use of the terms "first", "second", etc. to describe various elements is not intended to limit the positional relationship, chronological relationship or importance relationship of these elements. to distinguish one element from another. In some examples, a first element and a second element may refer to the same instance of the element, and in some cases, they may also refer to different instances based on contextual description.
[0049] The terminology used in describing the various described examples in this application is for the purpose of describing particular examples only and is not intended to be limiting. Unless the context clearly indicates otherwise, if the number of elements is not specifically limited, there may be one or more elements. In addition, the term "and / or" used in this application covers any one and all possible combinations of the listed items.
[0050] Below, will combine Figure 1A to Figu...
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The invention relates to a gene chip hybridization box, a gene chip set and a gene chip hybridization method. The gene chip hybridization box comprises a substrate, a cover plate and an spacer, wherein the substrate has a gene chip arrangement region to which a first surface of a gene chip is bondable; the cover plate is provided with a through hole, and the through hole is opposite to the gene chip arrangement region; the spacer is disposed between the substrate and the cover plate for generating a predetermined space between the substrate and the cover plate, and has an opening in a region facing the at least one gene chip arrangement region, thereby defining a gene chip accommodation space between the substrate and the cover plate; the predetermined interval is set such that, when the gene chip is fixed in the gene chip arrangement region, a second surface of the gene chip opposite to the first surface and including a probe region is spaced apart from a surface of the cover plate facing the second surface by 0.05 mm to 0.15 mm, and therefore, when hybridization liquid is injected through the through holes of the cover plate, the hybridization liquid can fully cover the second surface through capillary action.
Description
technical field [0001] The application relates to the field of biotechnology, and specifically relates to a gene chip hybridization box, a gene chip set and a gene chip hybridization method. Background technique [0002] Gene chip is an emerging biotechnology that can be used for DNA sequencing, identification of gene expression profiles, detection and analysis of gene mutants, etc. The basic principle of the gene chip is to design probes based on the known nucleotide sequence to hybridize with the labeled target nucleotide sequence, and perform qualitative and quantitative analysis by detecting the signal. The gene chip can integrate a large number of molecular recognition probes on the surface of a tiny substrate (silicon wafer, glass wafer, etc.), hybridize with the labeled sample, and obtain the genetic information of the sample through the detection and analysis of the hybridization signal. Parallel analysis of a large number of genes in-house for detection and analysi...
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IPC IPC(8): C12M1/34C12M1/00C12Q1/6837
CPCC12Q1/6837
Inventor 李峰何沛中戴小军简俊涛
Owner 生捷科技(杭州)有限公司