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Application of isothiazolinone as synergist of antibacterial drug

A technology of isothiazolinone and benzisothiazolinone, which is applied in the application field of synergists, can solve the problems of drug resistance and reduce the efficacy of antibacterial drugs, so as to reduce toxic and side effects, reduce drug costs, The effect of treating bacterial infection

Active Publication Date: 2020-10-30
南京艾力彼兽药研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when microorganisms come into contact with antibacterial drugs, they will also resist antibacterial drugs by changing their metabolic pathways or producing corresponding inactivated substances, thereby reducing the efficacy of antibacterial drugs and even developing drug resistance.

Method used

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  • Application of isothiazolinone as synergist of antibacterial drug
  • Application of isothiazolinone as synergist of antibacterial drug
  • Application of isothiazolinone as synergist of antibacterial drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In vitro combined susceptibility test (FIC assay) of methylisothiazolinone (MIT) combined with colistin sulfate (Colistin) against Escherichia coli and Salmonella.

[0023] 1 reagent

[0024] Methylisothiazolinone, colistin sulfate.

[0025] 2 strains

[0026] Clinical isolation of chicken Escherichia coli SD-02, clinical isolation of Salmonella pullorum AH-05.

[0027] 3 media

[0028] Nutrient Broth, Nutrient Agar.

[0029] 4 instruments

[0030] Constant temperature incubator, constant temperature shaker, micropipette.

[0031] 5 test method

[0032] 5.1 Determination of minimum inhibitory concentration (MIC)

[0033] (1) Firstly, the drug stock solution was diluted 10 times with broth to obtain a drug solution with a concentration of 256 μg / mL. Then carry out continuous two-fold dilution, take 100 μL of the diluted drug solution, and add them to the 1st to 11th wells of the microplate according to the concentration from high to low, and the corresponding con...

Embodiment 2

[0049] In vitro combined susceptibility test (FIC assay) of benzisothiazolinone (BIT) and enrofloxacin (EN) against Escherichia coli and Salmonella.

[0050] 1 reagent

[0051] Benzisothiazolinone, enrofloxacin.

[0052] 2 strains

[0053] Clinical isolation of chicken Escherichia coli SD-02, clinical isolation of Salmonella pullorum AH-05.

[0054] 3 media

[0055] Nutrient Broth, Nutrient Agar.

[0056] 4 instruments

[0057] Constant temperature incubator, constant temperature shaker, micropipette.

[0058] 5 test method

[0059] With embodiment 1.

[0060] 6 result judgment

[0061] With embodiment 1.

[0062] 7 results

[0063]

[0064] The FIC of benzisothiazolinone combined with enrofloxacin against Escherichia coli is 0.5.

[0065] The FIC of benzisothiazolinone combined with enrofloxacin against Salmonella was 0.5.

[0066] It can be seen from the above test results that benzisothiazolinone has a synergistic effect on the antibacterial effect of enrofloxa...

Embodiment 3

[0068] In vitro combined susceptibility test (FIC assay) of methylchloroisothiazolinone (CMIT) and neomycin sulfate (NEO) against Escherichia coli and Salmonella.

[0069] 1 reagent

[0070] Methylchloroisothiazolinone, neomycin sulfate.

[0071] 2 strains

[0072] Clinical isolation of chicken Escherichia coli SD-02, clinical isolation of Salmonella pullorum AH-05.

[0073] 3 media

[0074] Nutrient Broth, Nutrient Agar.

[0075] 4 instruments

[0076] Constant temperature incubator, constant temperature shaker, micropipette.

[0077] 5 test method

[0078] With embodiment 1.

[0079] 6 result judgment

[0080] With embodiment 1.

[0081] 7 results

[0082]

[0083] The FIC of the combination of methylchloroisothiazolinone and neomycin sulfate against Escherichia coli was 0.5.

[0084] The FIC of the combination of methylchloroisothiazolinone and neomycin sulfate against Salmonella was 0.5.

[0085] As can be seen from the above test results, methylchloroisothi...

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Abstract

The invention discloses an application of isothiazolinone as a synergist of an antibacterial drug, and belongs to the technical field of medicines. Experimental research shows that when isothiazolinone is combined with antibacterial drugs such as aminoglycoside, polypeptide and fluoroquinolone, the dosage of the antibacterial drugs can be reduced, the antibacterial effect of isothiazolinone on bacteria can be ensured under the condition that the dosage of the antibacterial drugs is reduced, and meanwhile, isothiazolinone has an obvious synergistic effect on clinically separated drug-resistantstrains.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of isothiazolinone as a synergist of antibacterial drugs. Background technique [0002] Isothiazolinone is a new type of high-efficiency broad-spectrum fungicide, which has the advantages of high efficiency, low toxicity, and environmental friendliness. Since it was developed for the field of fungicides, it has gradually replaced many toxic and low-efficiency mercury, etc. Fungicides are widely used in agriculture, industry and other fields (Li Shichang et al., Aging and Application of Synthetic Materials, 47(7), 120-123(2018)). It is widely used in anti-mildew and anti-corrosion in various fields of industrial production, and is also used as the main anti-bacterial preservative in some personal care products (daily cosmetics, washing products, etc.). The bactericidal mechanism of isothiazolinones is mainly an electrophilic active bactericide, relyin...

Claims

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Application Information

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IPC IPC(8): A61K31/425A61K31/428A61K45/06A61K38/12A61K31/496A61K31/7036A61P31/04
CPCA61K31/425A61K45/06A61K31/496A61K31/7036A61P31/04A61K2300/00Y02A50/30
Inventor 季辉万荣峰彭麟
Owner 南京艾力彼兽药研究所有限公司