Vancomycin-modified black phosphorus quantum dot antibacterial agent and preparation method and application thereof

A technology of vancomycin and antibacterial agent, which is applied in the application field of vancomycin-modified black phosphorus quantum dot antibacterial agent to prepare antibacterial products, which can solve problems such as toxic side effects, drug resistance, and high cost, and achieve biophase Good capacitance, excellent optical properties, and the effect of improving the antibacterial effect

Active Publication Date: 2020-10-30
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of antibiotics will bring a series of serious problems: drug resistance, toxic side effects, flora imbalance, etc.
However, in recent years, the development of new antibiotics has fallen into a very difficult predicament
On the one hand, the over-exploitation of soil resources makes it impossible to screen out new antibiotics from soil microorganisms, and synthetic chemistry only improves

Method used

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  • Vancomycin-modified black phosphorus quantum dot antibacterial agent and preparation method and application thereof
  • Vancomycin-modified black phosphorus quantum dot antibacterial agent and preparation method and application thereof
  • Vancomycin-modified black phosphorus quantum dot antibacterial agent and preparation method and application thereof

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preparation example Construction

[0033] Preferably, the specific steps of the preparation method are as follows: dissolve vancomycin hydrochloride and N-hydroxysuccinimide in DMSO, add 1-(3-dimethylaminopropyl)-3-ethyl Carbodiimide hydrochloride (EDC), mix and put in ice bath, continue to add N-BoC-(ethylenedioxy) diethylamine and N,N-diisopropylethylamine (DIPEA) Stir overnight, add acetone and centrifuge to obtain the precipitated part; obtain the sample by liquid phase separation, and process the sample sequentially through a mixture of dichloromethane, trifluoroacetic acid and ether; add black phosphorus quantum dot solution, and adjust the pH to 7~ 8 Obtain the black phosphorus quantum dots modified by vancomycin.

[0034] Preferably, the precipitation part is sequentially treated with a mixture of dichloromethane, trifluoroacetic acid and diethyl ether as follows: the sample is placed in a mixture of dichloromethane and trifluoroacetic acid and stirred in an ice bath for 0.8- After 1.2h, the solvent pa...

Embodiment 1

[0051] (1) 100 mg of black phosphorus crystals were ground into powder, and stored in 100 ml of N-methylpyrrolidone (NMP) solution under the protection of nitrogen. Take 10ml of black phosphorus NMP solution and sonicate for 15min. After the solution is evenly mixed, add 10ml of black phosphorus NMP solution, 90ml of NMP, and 100mg of NaOH into a 250ml Erlenmeyer flask, and stir for 6 hours under the protection of nitrogen at 140°C. . After the reaction, cool to room temperature. The reacted solution was centrifuged at 7000r for 20min to obtain the bottom black phosphorus and the supernatant. Then the supernatant was centrifuged at a rotating speed of 15100r for 20min to obtain the supernatant black phosphorus. The supernatant black phosphorus was washed 3 times and dried naturally to obtain the black phosphorus quantum dot antibacterial agent. Its TEM figure is as attached figure 1 shown.

[0052] (2) Prepare bacterial culture solution with the ratio of sodium chloride 10g...

Embodiment 2

[0055] (1) With step (1) in embodiment 1.

[0056] (2) Weigh 0.1040 g of vancomycin hydrochloride (70 μmol, 1 equivalent), and dissolve 0.0097 g of N-methyl succinimide (84 μmol, 1.2 equivalent) in 2 ml of dry DMSO, and place the mixture solution in ice water Cool the mixture to 0°C, add EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 84μmol, 1.2 equivalents) 0.161g, stir for 30min, put into ice water 0.0191 g of N-BoC-(ethylenedioxy)diethylamine (77 μmol, 1.1 equivalents) and 0.15 mol of DIPEA (860 μmol, 2.4 equivalents) were added to the mixture, and stirred overnight. Put the solution stirred overnight into a centrifuge tube, add acetone, centrifuge for 10 minutes, take out the supernatant, seal it and put it in the refrigerator, seal the precipitate and put it in the refrigerator to refrigerate. Dissolve the precipitate from cold storage and refrigerator with chromatographic methanol, centrifuge to take out the supernatant, separate the product by liquid...

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Abstract

The invention particularly relates to a vancomycin-modified black phosphorus quantum dot antibacterial agent and a preparation method and application thereof. Development of antibacterial drugs has the defects of long period and high cost, and the formation speed of strain drug resistance exceeds the research and development speed of drugs. In order to provide a drug with better antibacterial effect and reduced drug resistance, vancomycin modified black phosphorus quantum dots are adopted as an antibacterial active component, and vancomycin is bonded to the surfaces of the black phosphorus quantum dots through covalent bonds. Studies prove that the vancomycin-modified black phosphorus quantum dot has significantly improved antibacterial properties, is expected to become a first-line activecomponent of clinical antibacterial drugs, and has good development significance.

Description

technical field [0001] The invention belongs to the field of antibacterial technology, and in particular relates to a vancomycin-modified black phosphorus quantum dot antibacterial agent, a preparation method of the antibacterial agent and an application in preparing antibacterial products. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Various pathogenic bacteria are widely distributed in nature, and their spread and infection can cause a variety of diseases, thus threatening human health. Killing bacteria or preventing bacterial reproduction is the main idea of ​​current antibacterial. Among the traditional multi-class antibacterial drugs, antibiotics are a large clas...

Claims

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Application Information

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IPC IPC(8): A61K33/42A61K38/14A61K41/00A61K47/55A61K45/06A61P31/04A61K8/24A61K8/64A61Q17/00
CPCA61K33/42A61K38/14A61K41/0057A61K47/55A61K45/06A61P31/04A61K8/24A61K8/64A61Q17/005A61K2300/00
Inventor 杨燕美支晓钰田晓涵张晔牛伟华李然
Owner SHANDONG NORMAL UNIV
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