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A kind of ecl sensor for detecting single-stranded nucleic acid, its kit and its method

A single-stranded nucleic acid and sensor technology, applied in the field of biosensors, can solve the problems of destroying the microenvironment of the detection system, stability problems, and limited signal strength.

Active Publication Date: 2021-10-08
SOUTHWEST UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if these two systems add two co-reactants to synergize to enhance the ECL signal of quantum dots, the signal strength obtained is still very limited, and the extra co-reactants may destroy the microenvironment of the detection system, causing stability problems

Method used

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  • A kind of ecl sensor for detecting single-stranded nucleic acid, its kit and its method
  • A kind of ecl sensor for detecting single-stranded nucleic acid, its kit and its method
  • A kind of ecl sensor for detecting single-stranded nucleic acid, its kit and its method

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Embodiment

[0054] laboratory apparatus

[0055] The ECL intensity was recorded by the MPI-E multifunctional ECL system purchased from Xi'an Ruimai Analytical Instrument Co., Ltd. During the test, the voltage of the photomultiplier tube (PMT) was set to 800V, and the scanning speed was set to 0.3V / s. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) tests were performed with a CHI660E electrochemical workstation. For ECL and electrochemical tests (CV and EIS), the working electrodes are bare or modified glassy carbon electrodes (GCE, Φ=4.0mm), the auxiliary electrodes are platinum wires, and the reference electrodes are Ag / AgCl electrodes. (ECL detection) and saturated calomel electrode (SCE) (CV and EIS detection).

[0056] Characterization of nanomaterials using scanning electron microscopy. BG-verMIDI standard vertical electrophoresis instrument and Bio-Rad GelDoc XR+ system are used for native polyacrylamide gel electrophoresis and gel imaging respectively. ...

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Abstract

The invention discloses an ECL sensor for detecting single-stranded nucleic acid, a kit thereof and a method thereof, and relates to the field of biosensors. The method is based on a new ECL sensor, using PFO polymer nanoparticles as a single luminescent body, combined with the first nucleic acid; the first nucleic acid can be complementary combined with the nucleic acid to be tested to form a single-stranded structure, and then can be complementary combined with the second nucleic acid, The second nucleic acid is labeled with an enzyme for catalyzing the substrate to produce an enzymatic reaction product; the enzymatic reaction product can weaken the first signal value ECL-1 of the luminophore under the first scanning potential, and enhance the luminophore under the second scanning potential According to the second signal value ECL‑2 of the illuminant at different scanning potentials, the opposite changes of the two ECL signals at different scanning potentials can be obtained without adding co-reaction reagents, so as to The ratiometric ECL strategy realizes the detection of single-stranded nucleic acid and is of great value in biological analysis.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to an ECL sensor for detecting single-stranded nucleic acid, a kit thereof and a method thereof. Background technique [0002] Electrochemiluminescence (ECL) technology combines the advantages of electroluminescence and chemiluminescence, and has the properties of in situ and real-time detection. Ratiometric ECL analysis has attracted increasing attention due to the elimination of external interference and improved detection reliability. The currently reported ECL ratiometric strategies are usually constructed based on two different ECL luminophores, and utilize the resonance energy transfer between the two luminophores or between the luminophores and metal nanomaterials, or the competition between the ECL luminophores for the same co-reaction. Reagents such as H 2 o 2 or dissolved O 2 , and the change of the two ECL signals related to the concentration of the target is obtained, so as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N21/66C12Q1/6825
CPCC12Q1/6825G01N21/66G01N21/76C12Q2525/301C12Q2563/125C12Q2563/103C12Q2565/519C12Q2565/607
Inventor 陈时洪谭兴容刘堤杨国敏
Owner SOUTHWEST UNIV