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The role of azadirachtin in the preparation of drugs to promote the survival of ischemic ultra-long random skin flaps

A kind of azadirachtin and ultra-long technology, applied in the direction of drug combination, antipyretics, pharmaceutical formulations, etc., can solve the problems of skin flap tissue nutrition and metabolism disorders, failure of skin flap transplantation surgery, low survival rate of skin flaps, etc., to achieve Effects of reducing skin flap cell damage, increasing blood vessel density and blood flow, and reducing edema

Active Publication Date: 2021-10-22
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to ensure that the flap can survive smoothly during the transfer process, there are certain restrictions on the length and width ratio of the flap when designing the flap. The nutritional metabolism disorder of the distal part of the flap tissue leads to necrosis. Due to the low survival rate of the flap in the prior art, there are many restrictions on the transfer operation of the flap, and the flap transplantation operation cannot be applied to more scenarios

Method used

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  • The role of azadirachtin in the preparation of drugs to promote the survival of ischemic ultra-long random skin flaps
  • The role of azadirachtin in the preparation of drugs to promote the survival of ischemic ultra-long random skin flaps
  • The role of azadirachtin in the preparation of drugs to promote the survival of ischemic ultra-long random skin flaps

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Detection of skin flap survival area ratio and skin flap survival status

[0031] From 1 to 7 days after operation, the color, texture, tissue elasticity and hair growth or necrosis of rat skin flaps were observed daily with naked eyes and recorded. On the 7th day, under anesthesia, the rats in each group accurately measured the survival and necrosis area of ​​the skin flap with transparent paper, cut it into two parts, the living part and the dead part, and weighed them with electronic scales. Calculate the percentage of flap survival area [flap survival area transparent paper mass ÷ total flap surface area transparent paper mass × 100%].

[0032] Flap necrosis criteria: the color of the flap is black, the tissue retracts, the elasticity is poor, the texture is hard, and the cut tissue does not bleed. The pathological manifestations were tissue disintegration, nucleus disappearance, extensive infiltration of inflammatory cells, and focal hemorrhage.

[003...

Embodiment 2

[0034] Example 2: detection of skin flap neovascularization

[0035]Using laser Doppler blood flowmeter to detect the neovascularization of skin flap is a device that uses the principle of laser Doppler to monitor the blood perfusion of microcirculation in animals or human tissues. The laboratory of this subject has the instrument and supporting operating software to collect and analyze blood flow signals. On postoperative day 7, microcirculatory blood flow was measured using a laser Doppler flowmeter. Six rats per group were anesthetized and scanned using a Laserflo BPM (Vasamedic, Saint Paul, MN, United States) with a 15 cm x 15 cm area and 256 x 256 pixels. LDBF generally provides deeper penetration, enhancing visualization of small blood vessels beneath the tissue surface, and is ideal for angiogenesis assessment. The blood supply was visualized using strong LDBF signals (green, yellow and red), and its area was quantified using ImageJ software (NIH, Bethesda, MD, United...

Embodiment 3

[0039] Embodiment 3: Histological detection

[0040] Seven days after the operation, the rats were sacrificed, and tissue samples were cut from the middle of the flap, fixed in 4% (v / v) paraformaldehyde for 24 hours, and embedded in paraffin for transverse sectioning. Sections (4 μm thick) were mounted on poly-L-lysine-coated slides for H&E staining. Under a light microscope (20X magnification), the thickness of granulation tissue, edema and angiogenesis were observed. In addition, the neutrophil density and the number of microvessels per unit area ( / mm2) were calculated for each group.

[0041] The result is as Figure 7 As shown, the high-dose and low-dose groups of azadirachtin showed obvious subcutaneous fibroblast proliferation, thin granulation tissue, mild tissue edema, and obvious diffuse subcutaneous hemorrhage. The control flaps were thicker, had less fibroblast proliferation and neovascularization, and more severe edema and inflammatory cell infiltration ( Figu...

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Abstract

The present invention relates to the effect of azadirachtin in the preparation of drugs for promoting the survival of random skin flaps with ischemia. In the process of promoting the survival of random skin flaps with ischemia, it is taken orally. The dosage is 100 mg / kg or 200 mg / kg per day. 5-7 days is a course of treatment. Through a large number of animal experiments, it is concluded that the use of azadirachtin can significantly increase the survival area ratio of super long random skin flaps, reduce inflammation, improve antioxidant capacity, and greatly increase blood vessel density. Greatly promotes survival of ischemic random flaps.

Description

technical field [0001] The invention relates to a new application of azadirachtin, in particular to the role of azadirachtin in promoting the survival of ischemic ultra-long random skin flaps. Background technique [0002] In recent years, industry, transportation, energy, etc. have shown rapid development, and various traumas related to this, such as large-scale trauma and tissue defects, have become increasingly serious. This type of trauma usually leaves different degrees of deformity and dysfunction after repair, especially trauma deformity of the hands and face. As patients' requirements for perfect appearance and functional recovery are increasing day by day, the repair technology and treatment methods for wound problems have become an important consideration in medicine. Random flaps are most widely used in plastic surgery to repair tissue defects and deformities due to their greater flexibility in design. However, since the random flap lacks an arteriovenous system...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/343A61P9/10A61P39/06A61P29/00
CPCA61K31/343A61P9/10A61P29/00A61P39/06
Inventor 林丁盛何纪兵方妙杰马心怡李文杰刘莹莹
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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