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A reagent storage method in a test tube and an integrated test tube for reagent storage reaction detection

An in-test tube and test tube technology, which is applied in the field of reagent storage in test tubes and integrated test tubes for reagent storage and reaction detection. Simple operation, dense and uniform effect

Active Publication Date: 2021-01-29
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many kinds of reagents required for the four basic reactions, the operation steps are cumbersome, complex liquid manipulation and detection devices are required, the detection cost is high, and it is difficult to meet the needs of areas with backward medical infrastructure and on-site real-time detection; and reaction detection The long-term stable storage of reagents has always been one of the core problems in this field

Method used

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  • A reagent storage method in a test tube and an integrated test tube for reagent storage reaction detection
  • A reagent storage method in a test tube and an integrated test tube for reagent storage reaction detection
  • A reagent storage method in a test tube and an integrated test tube for reagent storage reaction detection

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Comparison scheme
Effect test

Embodiment 1

[0112] Embodiment 1. Sealed storage and release process of multiple reaction reagents:

[0113] Such as Figure 1a As shown, a first mixture 400a of the first phase change material and the first water phase reaction reagent is added to the test tube 100; wherein, the preferred first phase change material is paraffin wax, fatty acid and the like. The test tube 100 is sealed, heated to a specific temperature, so that the paraffin becomes liquid, and then the test tube 100 is placed in a centrifuge 500 for high-speed centrifugation. Alternatively, after the test tube 100 is sealed and placed on the centrifuge 500, the test tube 100 is centrifuged and heated to melt the paraffin. In the above process, due to the difference in density between the molten paraffin and the first reaction reagent, and the density of the paraffin is smaller than that of the reaction reagent, the mixture 400a of the molten paraffin and the first reaction reagent will be separated by liquid level under t...

Embodiment 2

[0122] Embodiment 2, nucleic acid direct amplification detection reaction:

[0123] Such as figure 2 Shown is an embodiment of the reagent storage reaction detection integrated test tube for nucleic acid direct amplification detection reaction of the present invention. The test tube 100 includes two parts: a test tube body 101 and a test tube cap 102 . The test tube cap 102 can seal the test tube main body 101 by screw thread or direct fit, so that the test tube 100 is completely sealed. The test tube 100 stores a reaction reagent 200 (polymerase chain reaction (PCR) system reagent) and a sealing layer 300 (paraffin sealing layer). Before using the test tube 100, open the test tube cap 102, add the nucleic acid template to be amplified and detected, and then close the test tube cap 102. Put the test tube into the PCR instrument, set a constant heating temperature, and melt the paraffin sealing layer, so that the nucleic acid template to be amplified and detected is mixed w...

Embodiment 3

[0124] Embodiment three, two-step nucleic acid amplification detection reaction:

[0125] Such as image 3 Shown is an embodiment of the reagent storage reaction detection integrated test tube used in the two-step nucleic acid isothermal amplification detection reaction of the present invention. The test tube 100 stores a first reaction reagent 200a (a system reagent for loop-mediated isothermal amplification (LAMP)) and a second reaction reagent 200b (a system reagent for recombinase polymerase amplification (RPA). The first sealing layer 300a (high melting point paraffin sealing layer) and the second sealing layer 300b (low melting point paraffin sealing layer) are respectively used to seal the two reagents. Before using the test tube 100 , open the test tube cap 102 , add the sample to be amplified and detected, and then close the test tube cap 102 . Put the test tube into the thermostatic temperature controller, set a constant heating temperature, first melt the low melt...

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Abstract

The invention discloses a reagent storage method in a test tube and an integrated test tube for reagent storage and reaction detection. The density of the change material is smaller than that of the reaction reagent, and the final solidified phase change material forms a dense thin layer above the reaction reagent to seal the reaction reagent. By repeating this method, the isolation and sealed storage of the required amount of reaction reagents can be completed. The reagent storage, reaction and detection integrated test tube prepared by this method can complete all reactions and final detection under airtight conditions through external control after adding the sample to be detected. The method provided by the invention greatly simplifies the complex equipment required for in vitro diagnostic response, is convenient, efficient, fast and low-cost, and is expected to promote the wide application of on-site instant detection.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a reagent storage method in a test tube and an integrated test tube for reagent storage reaction detection. Background technique [0002] The four basic detection types in the field of in vitro diagnostics, cell detection, biochemical detection, immune detection and nucleic acid amplification detection are widely used in clinical diagnosis and prognosis. However, there are many kinds of reagents required for the four basic reactions, the operation steps are cumbersome, complex liquid manipulation and detection devices are required, the detection cost is high, and it is difficult to meet the needs of areas with backward medical infrastructure and on-site real-time detection; and reaction detection The long-term stable storage of reagents has always been one of the core problems in this field. Contents of the invention [0003] In view of this, the present invention p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/14
CPCB01L3/50825
Inventor 徐友春李楠刘佳佳王会丽程京
Owner CAPITALBIO CORP