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Pan-cancer detection panel based on next-generation sequencing technology and application of detection panel

A second-generation sequencing technology and second-generation sequencing technology, applied in the field of pan-cancer detection panels

Pending Publication Date: 2020-11-24
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no relatively mature and comprehensive and accurate detection technology for pan-cancer using ctDNA technology in the market

Method used

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  • Pan-cancer detection panel based on next-generation sequencing technology and application of detection panel
  • Pan-cancer detection panel based on next-generation sequencing technology and application of detection panel
  • Pan-cancer detection panel based on next-generation sequencing technology and application of detection panel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035]Step 1. Take a patient's cancer tissue and blood samples to extract DNA. 1. Use the QIAsymphony automated platform to extract and purify DNA.

[0036] Step 2. Fragment screening

[0037] 2.1 Quantify the ctDNA concentration first. If the total amount of 20μL reaches 30ng or more, the normal process, if it does not reach 30ng, use more, concentrate (the maximum volume can be 30μL, and the water will decrease accordingly), etc., to reach between 20-30ng, and continue the experiment. If the concentration is too low, for example, the initial concentration is about 0.5, then at least 10ng of DNA must be added in the experiment after concentration.

[0038] 2.2 Take the AMPure XP magnetic beads out of the refrigerator and equilibrate to room temperature half an hour in advance; prepare fresh 80% EtOH in advance (take 40mL of absolute ethanol and add 10mL of nucleic acid-free water NFW).

[0039] 2.3 Shake the AMPure XP magnetic beads equilibrated to room temperature for no le...

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Abstract

The invention belongs to the technical field of pan-cancer polygene detection and discloses a pan-cancer detection panel based on a next-generation sequencing technology. Important exon regions and partial intron regions of 141 genes are enriched by utilizing a gene probe hybridization method, so that accurate detection is achieved; and more importantly, a detection object is patient DNA, and ctDNA is combined with a gene panel to serve as an examination object, so that targeted detection can be realized, and the gene detection cost of a patient and the operation difficulty of an experimentercan be reduced. Meanwhile, a polygene screening list is made into a probe, and high-risk genes are detected in a targeted mode through hybridization capture sequencing, so that gene mutation with guiding significance for diagnosis and treatment can be detected more efficiently, and meanwhile certain tumor patients can be helped to participate in clinical trials. The pan-cancer detection panel hasimportant guiding significance for clinical diagnosis and discovery of a target of targeted treatment.

Description

technical field [0001] The invention belongs to the technical field of pan-cancer multi-gene detection, and relates to a pan-cancer detection panel based on next-generation sequencing technology and its application. Background technique [0002] Cancer is the leading cause of death and disability in the world today. In the past decade, high-throughput sequencing and bioinformatics technology have made great progress, and the examination of cancer mutation genes has also gradually developed. Nowadays, more and more gene mutations have been confirmed to be associated with various forms of tumors, and more and more targeted drugs have been proven to be effective in treating certain types of cancer. In order to achieve precision medicine and apply the right drugs to the right patients, a large number of projects have begun to use next-generation sequencing technology for cancer-related genetic testing. Routine examinations need to extract normal tissue samples (or blood sample...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6874
CPCC12Q1/6886C12Q1/6874C12Q2600/156C12Q2600/112C12Q2600/118C12Q2535/122
Inventor 胡夕春郭伟剑陶中华张剑常建华张群岭张晓伟罗志国胡志皇曹军宁王佳蕾吴向华邵志敏黄薇胡欣施锦绣裴雨晨王云锦
Owner FUDAN UNIV SHANGHAI CANCER CENT
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