A short peptide hydrogel for submucosal injection of digestive tract and its application

A hydrogel and digestive tract technology, applied in the field of submucosal injection of short peptide hydrogel and its preparation, can solve the problems of delayed gelation, etc., and achieve good biocompatibility, rapid temperature and ion response characteristics , The effect of uniform and transparent appearance

Active Publication Date: 2021-05-28
CHINA JAPAN FRIENDSHIP HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, liquid injection may also flow out to surrounding tissues and blood vessels, or cause unexpected results such as delayed gelation due to the influence of complex physiological environments such as body fluid dilution

Method used

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  • A short peptide hydrogel for submucosal injection of digestive tract and its application
  • A short peptide hydrogel for submucosal injection of digestive tract and its application
  • A short peptide hydrogel for submucosal injection of digestive tract and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 14.23mg of Fmoc-YA powder and dissolve it in 1mL of PBS buffer (pH≈8), then vortex, sonicate and heat in a 70°C water bath for 6 minutes to completely dissolve into a clear and transparent solution, and finally stand at room temperature for 10 minutes Serve as a gel, continue to stand overnight to get 30mM Fmoc-YA injectable hydrogel, the physical picture is as follows figure 1 As shown, the appearance is clear and transparent, and the texture is uniform.

Embodiment 2

[0031] Weigh 6.92mg of RGD and dissolve it in 1mL of PBS buffer, then weigh 20.66mg of Fmoc-YL powder and add it into 1mL of PBS buffer, vortex, sonicate, and heat in a 75°C water bath for 5min to dissolve, then measure 0.5 mL of the above RGD solution and Fmoc-YL solution were mixed in equal proportions, and finally left at room temperature overnight to obtain a uniform and transparent injectable hydrogel co-assembled with Fmoc-YL (20 mM) and RGD (10 mM). Use the syringe filled with the above-mentioned hydrogel to inject regular geometric shapes on the surface of the glass slide, such as diagram 2-1 It is shown that the hydrogel has good injectability. In addition, in addition to the function of promoting cell adhesion, spreading, and proliferation of RGD, the present invention unexpectedly found that RGD itself has multiple groups that can provide hydrogen bonding sites, and can be co-assembled with Fmoc-dipeptide molecules , thereby enhancing the hydrogen bonding inside t...

Embodiment 3

[0033] Weigh 5.68mg of Fmoc-RGD and dissolve it in 1mL of PBS buffer, then weigh 20.02mg of Fmoc-FL powder and add it into 1mL of PBS buffer, dissolve it by shaking, ultrasonication and heating in a water bath at 80°C for 4min, then measure Take 0.5 mL of the above two solutions and mix them in equal proportions, and finally stand at room temperature overnight to obtain a uniform and transparent injectable hydrogel co-assembled with Fmoc-FL (20 mM) and Fmoc-RGD (5 mM). Afterwards, samples were taken and their microscopic morphology was observed with an atomic force microscope, as shown in image 3 The nanofiber network shown.

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Abstract

The invention relates to a short peptide hydrogel for submucosal injection of digestive tract, a preparation method and application thereof. The hydrogel is co-assembled by amphiphilic dipeptide and RGD or Fmoc-RGD, which has stimuli responsiveness, shear thinning and rapid self-healing properties, good biocompatibility, safety and environmental protection, simple operation, suitable for Large-scale production, applied to ESD surgery fillings, the uplift effect is good and the duration lasts for a long time, which improves the safety of the operation and reduces the difficulty and time of operation. In addition, it can also be used in the fields of biological nanomaterials, drug loading and controlled release, tissue repair and regeneration, cell scaffolds or tumor treatment.

Description

technical field [0001] The invention relates to technical fields such as biomedical materials and injectable gels, and in particular to a short peptide hydrogel for submucosal injection of the digestive tract and its preparation method and application. The hydrogel has stimuli responsiveness, shear thinning and Fast self-healing performance. Background technique [0002] Endoscopic submucosal dissection (ESD), as an advanced treatment technique, is mainly used for the clinical diagnosis and treatment of early cancers and precancerous lesions of the digestive tract. Compared with traditional invasive surgery, it has a high one-time resection rate, low local recurrence rate, less trauma, and can effectively relieve patients' pain. However, due to the thin wall of the digestive tract, the difficulty of resection is high and the risk of perforation is likely to occur. Therefore, it is necessary to inject fillers into the submucosa (under the lesion) to assist the operation. At...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K47/18A61K31/704A61P35/00A61L27/22A61L27/52
CPCA61K9/0019A61K9/06A61K31/704A61K47/183A61L27/227A61L27/52A61L2400/06A61P35/00
Inventor 李靖涛白硕任朋赵洪川
Owner CHINA JAPAN FRIENDSHIP HOSPITAL
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