A temperate phage vnem1 regulating coral flora and its application

Abstract

The invention discloses a mild phage VneM1 for regulating coral flora and its application. Phage Vibrio neocaledonicus bacteriophage VneM1, accession number: GDMCC NO.61215‑B1. The present invention is from coral symbiotic bacteria Vibrio neocaledonicus A new phage isolated from SCSIO 43009 - Phage Vibrio neocaledonicus bacteriophage VneM1, the phage VneM1 has a lytic effect on coral symbiotic Vibrio, regulates the composition of coral symbiotic microorganisms, and the polyp infection experiment proves that it can delay coral bleaching caused by temperature rise. Bacteriophage VneM1 is a temperate phage, usually integrated in the genome of lysogen SCSIO 43009 in the form of prophage. Phage can be produced spontaneously through lysogenic-cleavage transition, and environmental factors, such as SOS stress, can promote its lysogen-cleavage transition.

Description

[0001] The invention belongs to the field of marine biology, and in particular relates to a mild phage for regulating coral intestinal flora and its application. Background technique [0002] Coral reefs are a type of marine ecosystem rich in species diversity composed of corals, fish, algae and other marine animals and plants, as well as microorganisms. Coral reef ecosystems are highly productive, have important economic value, and play an important role in processes such as biogeochemical cycles. However, due to a combination of environmental pressures such as global warming and anthropogenic pressures, the productivity of coral reef ecosystems has declined dramatically over the past 30 years. The current situation of my country's coral reefs is particularly severe. The number of reef-building corals that make up the mainland and the hidden reefs of Hainan Island has dropped by 80%. According to reports, one of the important reasons for its sharp decline is the outbreak of ...

AI Technical Summary

Problems solved by technology

Phages may help corals resist disease by lysing pathogenic bacteria, such as using BA3 phage to specifically kill coral pathogenic bacteria Thalassomonas loyana , can effectively control the spread of pathogens in corals. However, due to the species-specificity of phage infection, BA3 phages cannot act on coral diseases caused by other coral pathogenic bacteria

Metamicrobiome study finds that some corals are born with beneficial phages that enhance coral resistance to disease, but these phages have not yet been identified

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