Small G protein Ras of oedaleus decorus asiaticus and coding gene and applications of small G protein Ras
A technology that encodes genes and locusts, applied in the fields of application, genetic engineering, plant genetic improvement, etc.
Active Publication Date: 2020-12-11
宁夏中微泰克生物技术有限责任公司
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Problems solved by technology
[0005] The invention provides a small G protein Ras of the Asian locust and its coding gene and application to solve the problem of locust disaster prevention and control
Method used
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preparation example Construction
[0110] Preparation of DNA template;
[0111] Synthesis of dsRNA;
[0112] The application of dsRNA in the control of Asian carcus.
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The present invention provides a small G protein Ras of oedaleus decorus asiaticus and a coding gene and applications of the small G protein Ras. The small G protein Ras of the oedaleus decorus asiaticus has an inhibition effect on growth and development of the oedaleus decorus asiaticus. The gene of the small G protein Ras of the oedaleus decorus asiaticus is firstly cloned from a body of theoedaleus decorus asiaticus, primers are designed for the gene of the small G protein Ras of the oedaleus decorus asiaticus, dsRNA for interfering the gene of the small G protein Ras of the oedaleus decorus asiaticus is synthesized, and the dsRNA is introduced into the oedaleus decorus asiaticus by using an injection method to perform RNAi on the gene of the small G protein Ras of the oedaleus decorusasiaticus. Results show that after the dsRNA is injected into the oedaleus decorus asiaticus, a survival rate, a growth rate, weight and an overall expressive force are all remarkably reduced, indicating that the gene of the small G protein Ras of the oedaleus decorus asiaticus plays an important role in growth and development processes of insects.
Description
technical field [0001] The present application relates to the field of biotechnology, in particular to a small G protein Ras of the Asian carcus and its coding gene and application. Background technique [0002] Grassland locust plagues have occurred year after year, seriously threatening agricultural and animal husbandry production. Due to the large area of grasslands and severe disasters, traditional locust control is mainly based on emergency prevention and control, and chemical pesticides are the main control agents. The use of chemical pesticides to control locust plagues not only endangers the environment, but pesticide residues are also a key topic of increasing concern to the people. Therefore, more and more biotechnology has been developed and applied to the control of locust plagues. [0003] Biologists have already used microbes to infect locusts with disease and death. Pathogenic microorganisms of locusts include microspores, fungi, bacteria, and viruses. M...
Claims
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Login to View More IPC IPC(8): C12N9/14C12N15/55C12N15/113C12N15/89A01N57/16A01P7/04
CPCC12N9/14C12N15/1137C12N15/89A01N57/16C12N2310/14
Inventor 涂雄兵张泽华李霜徐超民
Owner 宁夏中微泰克生物技术有限责任公司



