Method for in-vitro cloned embryo transplantation of pregnant sow

A technology for cloning embryos and sows, applied in medical science, veterinary instruments, animal delivery, etc., can solve the problems of difficulty in determining ovulation time, low cloning efficiency, restricting mass production applications, etc., to achieve mass production, increase success rate effect

Active Publication Date: 2020-12-18
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI +1
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cloning efficiency is still very low. Generally, the success rate of livestock is only 1% to 2% (number of surviving sons/cloned embryos). For pigs, the success rate is even lower than 1%, resulting in a huge waste of embryos and surrogate sows. Simultaneous implantation of a large number of embryo deaths has also raised more ethica

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for in-vitro cloned embryo transplantation of pregnant sow
  • Method for in-vitro cloned embryo transplantation of pregnant sow
  • Method for in-vitro cloned embryo transplantation of pregnant sow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0013] The estrus synchronous agent provided by the invention adopts tetraenestrone. The estrus synchronous agent acts as a negative feedback effect on the animal hypothalamus-pituitary gland-ovary complex, the pulse frequency of gonadotropin-releasing hormone is reduced, the secretion of follicle-stimulating hormone and luteinizing hormone is at the basic level, and the growth and development of follicles are inhibited . After the drug is stopped, the cycle starts again, and reaches the peak of estrus and ovulation after 5-7 days, and returns to the luteal phase from the 12th to 15th day, and the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for in-vitro cloned embryo transplantation of a pregnant sow. The method comprises the following steps: feeding an estrus synchronization agent to each sow at a fixedpoint every day, after continuously feeding each sow with the estrus synchronization agent for 18 days, performing intramuscular injection of PG600 5 days later, and selecting an individual with normal estrus for in-vitro cloned embryo transplantation 1 day later. According to the method, a proper microenvironment is provided for the pregnant sow after the cloned embryo is transplanted by virtue of feeding or injection of the estrus synchronization agent and the PG600, synchronism of the estrus, ovulation and embryo transplantation time of the pregnant sow is controlled, and the success rate of cloned embryo implantation and the pregnancy rate of the pregnant sow are greatly increased, so that batch production of cloned pigs is realized, and a foundation is laid for large-scale productionof the cloned pigs.

Description

technical field [0001] The invention belongs to the fields of estrus induction and cloned embryo transplantation, and in particular relates to a method for in vitro cloned embryo transplantation to surrogate sows. Background technique [0002] Somatic cell cloning technology has great application potential in the expansion and reproduction of excellent livestock breeds, the protection of endangered breeds, the production of transgenic animals, animal disease models, organ transplantation and other fields. However, the cloning efficiency is still very low. Generally, the success rate of livestock is only 1% to 2% (number of surviving sons / cloned embryos). For pigs, the success rate is even lower than 1%, resulting in a huge waste of embryos and surrogate sows. The death of a large number of embryos implanted at the same time has also aroused more ethical concerns. The estrus time and ovulation time of cloned embryo transplanted surrogate sows are difficult to determine, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61D19/04A61D7/00
CPCA61D19/04A61D7/00
Inventor 华再东郭帅毕延震任红艳肖红卫张立苹朱喆周昌胜
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap