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A method for evaluating the effect of drugs for preventing viral pneumonia

A technology for viral pneumonia and pneumonia, which is applied in the field of drug evaluation and screening, can solve the problems of large surgical trauma, high technical requirements, and heavy workload, and achieve the effects of short development cycle, high number of eggs, and strong comparability

Active Publication Date: 2022-04-08
北京环特智鱼优检生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, an ideal experimental animal model of pneumonia should be able to replicate the mechanism and consequences of human pneumonia, but so far no experimental animal model can completely replicate all the characteristics of human pneumonia. The pathophysiological characteristics can be replicated, such as abnormal ventilation function, decreased lung compliance, lung parenchymal injury and increased alveolar capillary membrane permeability, etc., and these animal models have complicated modeling operations, high technical requirements, and large surgical trauma. , low incidence of pneumonia, high complications and mortality, unstable model establishment, poor reproducibility, etc., long experimental cycle, high cost, and heavy workload, which are not conducive to the evaluation and screening of subsequent pneumonia treatment drugs
Others, such as in vitro cell models, lack the metabolic transformation of the drug in the whole organism and the circulation distribution in the body, and cannot reflect the real situation of the drug in the body

Method used

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  • A method for evaluating the effect of drugs for preventing viral pneumonia
  • A method for evaluating the effect of drugs for preventing viral pneumonia
  • A method for evaluating the effect of drugs for preventing viral pneumonia

Examples

Experimental program
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Effect test

Embodiment 1

[0053] A method for establishing an animal model of pneumonia, 30 tails of neutrophil transgenic green fluorescent zebrafish 5 days after fertilization were cultured in 3 mL of water, and the pneumonia-inducing agent poly(I:C) was injected into the zebrafish at a concentration of 100ng / tail, cultured at 28°C for 3 hours to obtain the model group;

[0054] At the same time, a normal control group was set up, and compared with the model group, the pneumonia-inducing agent was omitted.

Embodiment 2

[0070] A method for evaluating the effect of drugs for preventing viral pneumonia, the drug to be evaluated is indomethacin, comprising the following steps:

[0071] (1) Set up a normal control group: 30 tails of 5dpf transgenic neutrophil green fluorescent zebrafish were placed in 3 mL of water and incubated at 28°C for 3 hours at a constant temperature;

[0072] (2) Set up the model control group: place 10 zebrafish with the same specifications as in step (1) for culture in 3 mL of water, and inject 100 ng / tail of the pneumonia inducer poly(I:C) into the zebrafish;

[0073] (3) Set up the test sample group: 150 zebrafish with the same specifications as in step (1) were equally divided into 5 groups and placed in 3mL water for culture, and each group of zebrafish was injected with pneumonia inducer poly(I:C) 100ng / tail, and indomethacin was added in a water-soluble manner at the same time, the concentration of indomethacin was 7.5, 15, 30, 60 and 120 μM, and the activity st...

Embodiment 3

[0079]A method for evaluating the effect of drugs for preventing viral pneumonia, the drugs to be evaluated Shuanghuanglian Oral Liquid, Banlangen Granules and Compound Chuanxinlian Tablets, comprising the following steps:

[0080] (1) Set up a normal control group: 30 tails of 5dpf transgenic neutrophil green fluorescent zebrafish were placed in 3 mL of water and incubated at 28°C for 3 hours at a constant temperature;

[0081] (2) Set up the model control group: place 30 zebrafish of the same specification in step (1) for culture in 3 mL of water, and inject 100 ng / tail of the pneumonia-inducing agent poly(I:C) into the zebrafish, and cultivate according to the normal control group;

[0082] (3) Set up the test product group: 420 tails of zebrafish with the same specifications in step (1) are equally divided into 14 subgroups and placed in 3mL water for cultivation, wherein 5 subgroups are the Shuanghuanglian oral liquid group, 5 subgroups are the Radix Isatidis granule group...

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Abstract

The invention relates to the technical field of drug evaluation and screening. Aiming at the complicated modeling operation of existing animal models for evaluating pneumonia, which is not conducive to the evaluation and screening of subsequent pneumonia treatment drugs, a method for establishing an animal model of pneumonia is disclosed, which is reasonably used. A method for evaluating the effect of drugs on the prevention of viral pneumonia in the model. The pneumonia animal model is cultured by placing 5dpf zebrafish in water, and injecting the pneumonia inducer poly(I:C) into the zebrafish at a concentration of ≥50 ng / Tail established that when evaluating the drug, the evaluation indexes of inflammation subsiding effect, macrophage improving effect and relative gene RNA expression amount were used. The pneumonia model of the present invention can be used to evaluate the toxicity or efficacy of known drugs, and can also be used to screen unknown drugs, can accurately reflect the real situation of drugs in vivo, and can realize high-throughput screening in vivo or evaluate the effect of drugs on viral pneumonia. It has the advantages of reliability, rapidity, high efficiency, low cost and high cost performance.

Description

technical field [0001] The invention relates to the technical field of drug evaluation and screening, in particular to a method for evaluating the effect of drugs on preventing viral pneumonia. Background technique [0002] In non-bacterial pneumonia, viral pneumonia accounts for 25%-50%, and it is often aspiration infection. The main source of infection is the patient. It is transmitted through droplets and close contact. It occurs in eruptive viral infection, often accompanied by trachea-bronchial infection, mostly occurs in winter and spring, can be sporadic or popular, and is more common in infants, the elderly, and patients with existing chronic cardiopulmonary diseases. Among them, influenza virus is the most common pathogen of viral pneumonia in adults and the elderly, while viral pneumonia in infants and young children is often caused by respiratory syncytial virus infection. Others such as parainfluenza virus, cytomegalovirus, coronavirus, adenovirus, rhinovirus an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/13A61K49/00
CPCA01K61/13A61K49/0008Y02A40/81
Inventor 黄燕烽吴雨桐朱晓宇徐懿乔郭胜亚李春启
Owner 北京环特智鱼优检生物科技有限公司
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