Method for detecting vitamins in formula food for special medical purposes

A technology of formula food and detection method, applied in the field of vitamin detection, can solve the problems of high detection limit, poor repeatability, and unstable enzyme-linked immunosorbent assay results.

Inactive Publication Date: 2020-12-22
特康药业集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The lower detection limit of HPLC method is too high, and the detection result of enzyme-linked immunoassay is not stable enough. Although the detection result of classical microbial method is accurate an

Method used

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Embodiment Construction

[0038] A method for the detection of vitamins in formula foods for special medical purposes:

[0039] principle:

[0040] After the sample is extracted and other pre-treatments, it is subjected to C 18 Separation by chromatographic column, detection by high performance liquid chromatography-fluorescence detector, quantitative determination of the content of vitamins (vitamin B1, B2, B6, nicotinamide, nicotinic acid, folic acid) by external standard method.

[0041] Reagents and materials:

[0042] The reagents used in this method are analytically pure, and the water is the first-grade water specified in GB / T6682.

[0043] Reagent:

[0044] Sodium octane sulfonate (C 8 h 17 NaO 3 S), glacial acetic acid (C 2 h 4 o 2 ), triethylamine (C 6 h 15 N) (chromatographically pure), methanol (CH 4 O) (chromatographically pure), hydrochloric acid (HCl), sodium hydroxide, amylase (enzyme activity ≥ 1.5U / mg).

[0045] Reagent preparation

[0046] Hydrochloric acid solution (...

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Abstract

The invention discloses a method for detecting vitamins in formula food for special medical purposes, and relates to the technical field of vitamin detection, and the method comprises the following steps: preparing standard solutions: preparing 1 mg/mL of vitamin B6 standard stock solution, 1 mg/mL of vitamin B1 standard stock solution, 1 mg/mL of vitamin B2 standard stock solution, 1 mg/mL of nicotinic acid nicotinamide standard stock solution, and leaf standard stock solution; preparing mixed standard work solutions: accurately sucking the standard stock solutions, and fixing the volumes bya mobile phase so as to obtain a series of curve solutions, wherein the concentrations of the curve solutions are 1 microgram/mL, 2 micrograms/mL, 5 micrograms/mL, 10 micrograms/mL and 20 micrograms/mL respectively; preparing a sample: firstly preparing a sample solution, and then preparing a to-be-detected solution by using the sample solution; and analyzing the result: injecting the sample solution into a high performance liquid chromatograph to obtain the corresponding peak area of each component, and obtaining the concentration of each vitamin component in the to-be-tested sample solutionaccording to a standard curve, wherein the content of each vitamin component in the sample is calculated according to the following formula: Xi=([rho]*V)/m*100/1000.

Description

technical field [0001] The invention relates to the technical field of vitamin detection, in particular to a method for detecting vitamins in formula food for special medical purposes. Background technique [0002] Vitamins are organic compounds necessary for life-maintaining physiological functions. The content of vitamins in the natural ingredients of food is very small. Although they are trace amounts, they are essential to the normal physiological functions of the matrix. If they are missing or insufficient, they will cause special deficiency syndromes. At present, 13 substances or substance groups are generally regarded as vitamins, which can be divided into fat-soluble vitamins and water-soluble vitamins according to their solubility. Methods for the determination of water-soluble vitamins include microbial methods, liquid chromatography, high performance liquid chromatography-mass spectrometry, and the like. Among them, the traditional microbial method has high sensi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/36G01N30/74G01N30/8679G01N2030/042
Inventor 张灵燕王伟伟张相玲赵慧丁元康山秀英戚海波
Owner 特康药业集团有限公司
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