A kind of chromogenic solid culture medium of Nocardia japonica and its preparation method and application

A technology of solid culture medium for Nocardia spp., applied in the field of chromogenic solid medium for Nocardia spp. and its preparation, which can solve the problems of difficult to achieve expected results, high separation cost, and difficult separation. , to achieve the effect of convenient observation, high sensitivity and strong specificity

Active Publication Date: 2022-06-17
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the molecular weight of the quorum effect substance and its similarity, the separation is difficult and the separation cost is high
In addition, the composition of bacteria quorum effect substances is complex, and it is difficult to achieve the expected effect by artificially adding a single substance

Method used

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  • A kind of chromogenic solid culture medium of Nocardia japonica and its preparation method and application
  • A kind of chromogenic solid culture medium of Nocardia japonica and its preparation method and application
  • A kind of chromogenic solid culture medium of Nocardia japonica and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0043] This embodiment provides a color developing solid culture medium of Nocardia amberjack, and its preparation method comprises the following steps:

[0044] S1. Inoculate Nocardia amberjack with brain-heart infusion broth (BHI) liquid medium (purchased from Beijing Soleibo Technology Co., Ltd.), and cultivate to logarithmic growth phase (OD=0.5~0.6), and then by Centrifuge, take the supernatant, sterilize it through a bacterial filter (the pore size of the filter membrane is 0.22 μm), and collect the filtrate to obtain the Nocardia amberjack culture supernatant.

[0045] S2, take by weighing 30g of brain heart extract broth culture medium powder (purchased from Beijing Soleibao Technology Co., Ltd.), 15g of agar powder, 0.5g of esculin, 0.05g of ferric citrate, 0.005g of antibiotic composition, 900g of distilled water, 180 mL of the above Nocardia amberjack culture supernatant was used for later use. Wherein, the antibiotic composition is obtained by mixing 0.005 g of ce...

Embodiment 2

[0049] This embodiment provides a color developing solid culture medium of Nocardia amberjack, and its preparation method comprises the following steps:

[0050] S1. Inoculate Nocardia amberjack with brain-heart infusion broth (BHI) liquid medium (purchased from Beijing Soleibo Technology Co., Ltd.), and cultivate to logarithmic growth phase (OD=0.5~0.6), and then by Centrifuge, take the supernatant, sterilize it through a bacterial filter (the pore size of the filter membrane is 0.22 μm), and collect the filtrate to obtain the Nocardia amberjack culture supernatant.

[0051] S2, take by weighing 45g of brain heart extract broth culture medium powder (purchased from Beijing Soleibao Technology Co., Ltd.), 25g of agar powder, 1.5g of esculin, 0.5g of ferric citrate, 0.09g of antibiotic composition, 1100g of distilled water, 55 mL of the above-mentioned Nocardia amberjack culture supernatant was used for later use. Wherein, the antibiotic composition is obtained by mixing 0.02 ...

Embodiment 3

[0055] This embodiment provides a color developing solid culture medium of Nocardia amberjack, and its preparation method comprises the following steps:

[0056] S1. Inoculate Nocardia amberjack with brain-heart infusion broth (BHI) liquid medium (purchased from Beijing Soleibo Technology Co., Ltd.), and cultivate to logarithmic growth phase (OD=0.5~0.6), and then by Centrifuge, take the supernatant, sterilize it through a bacterial filter (the pore size of the filter membrane is 0.22 μm), and collect the filtrate to obtain the Nocardia amberjack culture supernatant.

[0057] S2, weigh 35g of brain-heart extract broth culture medium powder (purchased from Beijing Soleibao Technology Co., Ltd.), 18g of agar powder, 0.7g of escin, 0.4g of ferric citrate, 0.06g of antibiotic composition, 1000g of distilled water, 100 mL of the above Nocardia amberjack culture supernatant was used for later use. Wherein, the antibiotic composition is obtained by mixing 0.005 g of cefoxidine sodiu...

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Abstract

The present invention is applicable to the field of fish pathogenic microbiology, and provides a chromogenic solid medium for Nocardia amberiella and its preparation method and application. The chromogenic solid medium includes the following components: Brain heart extract broth Medium powder, agar powder, esculin, ferric citrate, antibiotic composition, distilled water, culture supernatant of Nocardia japonica. The present invention can be prepared by adding escin, ferric citrate, culture supernatant of Nocardia japonica in logarithmic growth phase and antibiotic composition to the basic formula of brain-heart extract broth agar medium. A culture medium with the characteristics of short separation time, high sensitivity, high purity of isolated bacteria and simple preparation method was obtained. The use of this medium for the isolation of Nocardia spp. has the characteristics of high separation efficiency, high separation purity, and convenient observation. It can be effectively applied to the isolation of Nocardia spp. from clinical samples.

Description

technical field [0001] The invention belongs to the field of fish pathogenic microbiology, and in particular relates to a color developing solid culture medium of Nocardia amberjack and a preparation method and application thereof. Background technique [0002] Nocardia amberjack is the main pathogen of nocardiosis in aquaculture, widely distributed in the ocean, soil, activated sludge, decaying vegetation and animal excrement. It can mainly cause white nodules in the internal organs of fish, resulting in slow movement of fish, decreased appetite, low immunity, and then death. At present, the main fishes reported in the literature are: largemouth bass, oval pomfret, snakehead and so on. Nocardia amberjack is a facultative aerobic gram-positive bacteria, the content of which is not high in water. When aquatic animals are weak and their immunity is low, Nocardia amberjack can pass through the gills or wounds. Infect. The disease has a long incidence period, high infection r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/365
CPCC12N1/20
Inventor 夏立群侯素莹鲁义善王文基陈国权
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
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