Method for high-cell-biomass production of carotenoids

Abstract

The invention discloses a method for high-cell-biomass production of carotenoids. The method comprises the following steps: with leftovers of canned yellow peaches as main raw materials, conducting microwave color fixation, freeze-breaking and biological enzymolysis, so as to obtain fruit juice which is relatively high in content of the carotenoids; adjusting components so as to obtain a fermentation culture medium, gradually inoculating the fermentation culture medium with an oceanic red yeast which can achieve high-yield production of the carotenoids, and conducting variable-temperature fermentation so as to promote the maximum proliferation of the oceanic red yeast; conducting ultrahigh-pressure homogenizing on a fermentation broth, and conducting vacuum centrifuging, and demulsification and separation so as to obtain an emulsion; and conducting extracting on the emulsion, and conducting separating and purifying so as to obtain the finished product carotenoids, wherein the content of the cell biomass of the fermentation broth is 38-52g / L and the yield of the carotenoids is 47-61mg / L. The preparation method is simple, short in cycle, high in efficiency, energy-saving and environmentally friendly, and the preparation method, which adopts a low-temperature biological processing technology in a whole course, reduces production cost, eliminates the hidden trouble of environmental pollution and lays a solid foundation for the sustainable development of the yellow peach deep processing technical field; therefore, the preparation method has positive economic and social development significance.

Description

technical field [0001] The invention relates to the preparation of carotenoids, in particular to a method for producing carotenoids with high cell biomass. Background technique [0002] Carotenoids are a general term for a group of fat-soluble pigments with antioxidant properties. As a class of important biologically active substances in humans and animals, carotenoids have important functions such as enhancing the body's immunity, anti-radiation, anti-tumor, and treating photosensitivity diseases. , It has a wide range of applications in food, cosmetics, medicine and feed industries. At present, carotenoids have been recognized as Class A nutritional pigments by international organizations such as FAO, European Community and WHO, and in recent years, the industrialization development of carotenoids is gradually taking shape. [0003] The industrial production method of carotenoids can be chemical synthesis or plant extraction. The chemical synthesis of carotenoids is not ...

AI Technical Summary

Problems solved by technology

[0014] The technical problem solved by the present invention is to overcome the defects of the existing fermentation preparation of carotenoids, and use the leftovers of canned yellow peaches as the main raw material to obtain fruit juice with high carotenoid content through microwave solidification, freezing and crushing, and biological enzymatic hydrolysis , after adjusting the components to obtain the fermentation medium, inoculate the marine red yeast with high carotenoid production into it for staged inoculation and variable temperature fermentation, so as to maximize the proliferation of marine red yeast, and the fermentation liquid is homogenized by ultra-high pressure, vacuum centrifuged, and demulsified After separation, the emulsion is obtained, and the emulsion is extracted, separated and purified to obtain the finished carotenoids