Method for high-density cultivation for lactobacillus by using bone collagen polypeptide powder

A technology for high-density culture of Lactobacillus sake, applied in the field of high-density culture of lactic acid bacteria, to achieve good effect, high activity, and prolong the logarithmic growth phase

Inactive Publication Date: 2011-04-20
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cultivation using the above technology has problems such as high cost and difficult operation, and it is even more difficult to popularize and apply it in food enterprises

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for high-density cultivation of lactic acid bacteria by using bone collagen polypeptide powder. The method uses Lactobacillus Sake as a strain, and first uses a lactic acid bacteria basal medium (MRS medium) to activate and expand Lactobacillus Sake. Cultivate, then inoculate into the fermentation medium according to the inoculation amount of 3%, and ferment and cultivate at 37°C for 14 hours. During the fermentation and cultivation, add 2mol / L NaOH solution to control the pH value to 6.8, and collect it by centrifugation after the cultivation. The thalline is added to the protein protection agent to dissolve (the mass ratio of the collected thalline to the protein protection agent for dissolving the thalline is 1:4), and finally the dry powder type lactic acid bacteria starter is obtained by vacuum freezing and drying.

[0017] The components and mass fractions of the above-mentioned fermentation medium are as follows: collagen polypeptide powder 0.5%, yeast ex...

Embodiment 2

[0023] A method for high-density cultivation of lactic acid bacteria by using bone collagen polypeptide powder. The method uses Lactobacillus sake as a bacterial species. First, the lactic acid bacteria basal medium (MRS medium) is used to activate and expand the Lactobacillus sake. Inoculate 3% of the inoculum into the fermentation medium, and ferment at 37°C for 12 hours. During the fermentation process, add 2mol / L NaOH solution to control the pH value to 6.5. Dissolve in the protein protectant (the mass ratio of the collected thalline to the protein protectant for dissolving the thalline is 1:4), and finally vacuum freeze and dry to obtain a dry powder lactic acid bacteria starter.

[0024] The components and mass fractions in the above fermentation medium are as follows: collagen polypeptide powder 1%, yeast extract 0.5%, glucose 0.5%, KH 2 PO 40.3%, Tween 800.5%, tomato juice 5%, sodium acetate 0.5%, sodium citrate 0.2% and the rest of water, adjust the initial pH value ...

Embodiment 3

[0027] In this example, only the mass fraction of collagen polypeptide powder added to the fermentation medium in Example 1 was adjusted to 1.5%, and the rest of the process steps and process parameters were the same as in Example 1.

[0028] The number of live bacteria of Lactobacillus sake in the dry powder starter prepared by the culture method of this embodiment is 8.8×10 10 CFU / g, the number of viable bacteria in the fermentation broth was 8.9×10 9 CFU / mL.

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Abstract

The invention discloses a process for using bone collagen polypeptide powder to perform high-density culture to the lactic acid bacteria. The method takes the lactic acid bacteria as bacteria. The method includes: firstly suing the lactic acid bacteria basic medium to perform activating and expanding culture to the bacterial, and then inoculating to the fermentation medium for fermentation culture 12-18h, collecting the thalli and adding it in the protein protective agent for dissolving, and finally obtaining the completed product by vacuum freezing and drying. Bone collagen polypeptides powder is added in the fermentation medium. The inventive culture method has advantages of low cost, simple operation, etc., and can obtain lactobacillus completed product with high activity and high concentrations.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to a method for high-density cultivation of lactic acid bacteria. Background technique [0002] Lactic acid bacteria are a general term for a large group of bacteria that can ferment lactose and produce mainly lactic acid. They are mainly distributed in the intestines of humans and animals. Substances that resist the invasion of pathogenic bacteria in the intestinal tract to their hosts, and have antagonistic effects on common pathogenic bacteria (such as Shigella, Salmonella typhi, pathogenic Escherichia coli, Staphylococcus, etc.). In addition, lactic acid bacteria also have the effect of preventing and treating constipation, diarrhea and gastrointestinal disorders. It produces a large amount of lactic acid, which can promote intestinal wall peristalsis, help digestion, synthesize vitamins, amino acids and other substances in the intestinal tract, and at the same time he...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/225
Inventor 李宗军李珂王远亮李罗明王传花谢静
Owner HUNAN AGRICULTURAL UNIV
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