Preparation method and application of zinc organic framework composite material for photoresponsively releasing vancomycin

A vancomycin, light-responsive technology, applied in the field of biomedical antibacterial, can solve the problem of damage to healthy tissues, and achieve the effect of short time-consuming, low cost, excellent photothermal/drug synergistic antibacterial effect

Inactive Publication Date: 2021-01-01
THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Lower temperatures are insufficient for short periods of time to completely eliminate stubborn bacterial infections, and higher

Method used

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  • Preparation method and application of zinc organic framework composite material for photoresponsively releasing vancomycin
  • Preparation method and application of zinc organic framework composite material for photoresponsively releasing vancomycin
  • Preparation method and application of zinc organic framework composite material for photoresponsively releasing vancomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for preparing a zinc organic framework composite material that releases vancomycin in response to light, and the specific preparation method includes the following steps:

[0043] Step 1: Dissolve vancomycin (5 mg) and zinc nitrate hexahydrate (0.2 g) in 5 mL of water, and stir the mixture for 5 minutes;

[0044] Step 2: Add 10 mL of 2-methylimidazole solution dropwise to the mixture obtained above, stir again for 15 minutes, collect by centrifugation and use H 2 O and methanol were mixed and washed three times, and then dried under vacuum to obtain Van@ZIF-8;

[0045] Step 3: Suspend 5 mg Van@ZIF-8 in 30 mL Tris-HCl buffer (10 mM, pH 8.5). After ultrasonic stirring for 5 minutes, 5 mg of dopamine hydrochloride was added and stirred at room temperature for 2 hours.

[0046] Step 4: Use H after centrifugation 2 O and methanol were mixed and washed three times, and then dried under vacuum to obtain the final product Van@ZIF-8@PDA.

Embodiment 2

[0048] The present invention also provides the application of zinc organic framework composite materials that release vancomycin in response to light. According to the method prepared in Example 1, a zinc organic framework composite nanomaterial that releases vancomycin under near-infrared light is prepared. Namely Van@ZIF-8@PDA.

[0049]The application of the antibacterial composite material, the photoresponsive release of vancomycin zinc organic framework composite material, can control the release of vancomycin under near-infrared light stimulation and reduce the working concentration of vancomycin.

[0050] The application of the antimicrobial composite material, Zn-organic framework composite material with photoresponsive release of vancomycin, has remarkable antibacterial activity under near-infrared light, and can effectively fight against the biofilm of vancomycin-mediated drug-resistant Staphylococcus aureus Mu50 active.

[0051] The application of this antimicrobial...

Embodiment 3

[0053] like Figure 1-4 As shown, the Van@ZIF-8@PDA nanocomposite prepared by the present invention is characterized:

[0054] like figure 1 As shown, in accordance with figure 1 Schematically, the photoresponsive release of vancomycin zinc-organic framework composite material Van@ZIF-8@PDA was prepared;

[0055] like figure 2 As shown, the morphology of Van@ZIF-8@PDA was characterized by electron microscopy, and the results showed that the nanoparticles were spherical;

[0056] like image 3 As shown, the hydrated particle size (DLS) measurement of Van@ZIF-8@PDA shows that the average particle size measured by Van@ZIF-8@PDA is about 171.6 nm;

[0057] like Figure 4 As shown, the surface potential of Van@ZIF-8@PDA was measured, and the measured surface potential was −9.3 ± 0.46 mV. These results confirmed the successful synthesis of Van@ZIF-8@PDA nanomaterials.

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Abstract

The invention discloses a preparation method and application of a zinc organic framework composite material for photoresponsively releasing vancomycin. The composite material is formed by encapsulating vancomycin in a zinc organic framework and further modifying the surface of the zinc organic framework with polydopamine, and the material is called Van @ ZIF-8 @ PDA for short, wherein Van is vancomycin, ZIF-8 is the zinc organic framework, and PDA is polydopamine. The preparation method comprises the following steps of: firstly, dissolving vancomycin and zinc nitrate hexahydrate in an aqueoussolution, and mixing and stirring; then dropwise adding a 2-methylimidazole solution, stirring again, and then performing centrifugal washing and vacuum drying to obtain the zinc organic framework (Van @ ZIF-8 @ PDA) encapsulating vancomycin; and then adding dopamine into a Tris-HCl solution of Van @ ZIF-8, mixing and stirring, and performing centrifugal washing and vacuum drying to obtain the Van@ ZIF-8 @ PDA composite material. The invention belongs to the field of antibacterial drugs, and the composite material as a novel antibacterial drug has a remarkable inhibiting effect on vancomycin-mediated drug-resistant staphylococcus aureus Mu50 and a biological membrane thereof, can remarkably reduce the working concentration of antibiotics, and has a huge potential in treatment of drug-resistant bacteria.

Description

technical field [0001] The invention belongs to the field of biomedical antibacterial, and relates to a preparation method of a zinc organic framework composite material that releases vancomycin in response to light, and an application as an antibacterial drug. Background technique [0002] Antibiotics are the most common treatment for preventing and treating bacterial infections. But the abuse of antibiotics will lead to the emergence and prevalence of drug-resistant bacteria. Furthermore, bacterial infection and biofilm formation is a serious clinical problem. Therefore, alternative therapeutic strategies targeting bacterial infections and biofilms are urgently needed to reduce the emergence of drug resistance. [0003] In recent years, nanomaterials with high drug-loading capacity and other characteristics are becoming new drug preparations in the field of antibacterial. Compared with traditional antibiotics, nanomaterials are less likely to induce bacterial resistance...

Claims

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Application Information

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IPC IPC(8): A61K38/14A61K41/00A61K47/59A61P31/04
CPCA61K38/14A61K41/0052A61K47/59A61P31/04A61K2300/00
Inventor 李家斌
Owner THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
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