Olive leaf element derivative and preparation method and application thereof

A technology of olive leaf element and derivatives, applied in the field of antibacterial agents, can solve problems such as insufficient research, and achieve the effect of excellent antibacterial performance

Active Publication Date: 2021-01-05
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, other olivine oleifolins in S. hygroscopicus are not well studied

Method used

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  • Olive leaf element derivative and preparation method and application thereof
  • Olive leaf element derivative and preparation method and application thereof
  • Olive leaf element derivative and preparation method and application thereof

Examples

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preparation example Construction

[0048] The present invention provides the preparation method of the olive folin derivative described in the above technical scheme, comprising the following steps:

[0049] (1) Carry out seed culture to Streptomyces hygroscopicus LP-93, obtain LP-93 seed liquid;

[0050] (2) Inoculate the LP-93 seed liquid in the fermentation medium to carry out fermentation culture, adjust the pH value of the obtained fermentation liquid to 3, centrifuge, air-dry the obtained precipitate and pulverize to obtain LP-93 fermented dry bacteria body powder;

[0051] (3) carrying out ethanol extraction of the LP-93 fermented dry cell powder, concentrating the obtained extract, dissolving the obtained concentrate with methanol and filtering, concentrating the obtained filtrate to obtain the LP-93 mycelium extract;

[0052] (4) mixing the LP-93 mycelium extract with ethyl acetate to obtain the soluble fraction F1;

[0053] (5) The soluble part F1 is subjected to silica gel column chromatographic se...

Embodiment 1

[0091] (1) Place Streptomyces hygroscopicus LP-93 (China Pharmaceutical Microbiological Strain Preservation and Management Center) on a slant medium and culture it on a slant at 28°C for 7 days to obtain a bacterial slant, and inoculate a 1cm×1cm bacterial slant by the method of digging In a 500mL Erlenmeyer flask equipped with 50mL seed medium, shake the seeds on a shaking table at 28°C and 220r / min for 48h to obtain LP-93 seed liquid; wherein, the composition of the slant medium: asparagine 0.05wt%, K 2 HPO4 0.05wt%, glucose 1wt%, agar 1.2wt% and surplus water; The composition of seed culture medium is: 3.0wt% soybean cake powder, 3.5wt% glucose, 0.3wt% ammonium sulfate, 0.15wt% calcium carbonate and surplus water.

[0092] (2) Inoculate the LP-93 seed solution obtained in step (1) into a 500mL Erlenmeyer flask with 50mL fermentation medium at an inoculum size of 10% by volume fraction, and culture on a shaking table at 25°C and 220r / min 96h to obtain LP-93 fermented liqui...

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Abstract

The invention provides an olive leaf element derivative and a preparation method and application thereof, and belongs to the technical field of antibacterial agents. The olive leaf element derivativehas a structure shown in the formula I, wherein R is methyl or ethyl. The olive leaf element derivative is novel in structure and excellent in antibacterial performance on staphylococcus epidermidis,staphylococcus aureus, staphylococcus hominis and enterococcus faecalis.

Description

technical field [0001] The invention relates to the technical field of antibacterial agents, in particular to an olive leaf derivative and its preparation method and application. Background technique [0002] Olivine (English name: Elaiophylin, Azalomycin B, Gopalamicin, or Salbomycin) is a class of sixteen-membered ring macrocyclic dilactone antibiotics with double rotational symmetry, which has a wide range of antibacterial, antiparasitic, and promotion of rumen animal growth. biological activity. [0003] Streptomyces hygroscopicus is a kind of actinomycetes with broad antibacterial spectrum and stable genetic traits, which can produce nigericins, oleifolins, geldanamycins, rapamycins, etc. Type metabolites, with antibacterial, immunosuppressive and other biological activities. Liu Yufeng et al. (Liu Yufeng et al. Isolation and structural identification of oleifolin derivatives in the mycelia of Streptomyces hygroscopicus LP-93. Journal of Jining Medical College. 2020,4...

Claims

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Application Information

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IPC IPC(8): C07H17/04A61K31/7048A61P31/04A61P31/10
CPCA61P31/04A61P31/10C07H17/04C12P19/60
Inventor杨兆勇金媛媛刘玉凤王保国樊帅吕广新张茜
OwnerMEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI