A kind of thrips attractant and insecticide combined trapping and killing method
A technology of attractants and insecticides, applied in the direction of insecticides, attracting pests, botanical equipment and methods, etc., can solve problems affecting the trapping effect, achieve environmental safety, and prevent secondary escape from causing damage
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Embodiment 1
[0027] One, the preparation method of thrips attractant
[0028] 1.1 Preparation of 100 μg / ml single component solution
[0029] Preparation of 100 μg / ml methyl nicotinate solution: Add 10 μl of the pre-prepared 10 μg / μl methyl nicotinate standard compound into 990 μl of liquid paraffin, and mix evenly;
[0030] Preparation of 100 μg / ml trans-β-farnesene solution: Add 10 μl of the pre-prepared 10 μg / μl trans-β-farnesene standard compound into 990 μl of liquid paraffin, and mix evenly;
[0031] Preparation of 100 μg / ml octanal solution: Add 10 μl of the previously prepared 10 μg / μl octanal standard compound into 990 μl of liquid paraffin, and mix evenly;
[0032] Preparation of 100 μg / ml 3-carene solution: Add 10 μl of the previously prepared 10 μg / μl 3-carene standard compound into 990 μl of liquid paraffin, and mix evenly;
[0033] Preparation of 100 μg / ml isoeugenol solution: Add 10 μl of the previously prepared 10 μg / μl isoeugenol standard compound into 990 μl of liquid p...
Embodiment 2
[0049] The Y-type olfactory instrument was used to measure the directional behavior response of Thrips palmi to the compound (improved by our laboratory based on the basic principle of the olfactory instrument, the authorized patent number is: ZL201020239767.7). The main arm of the Y-shaped tube is 20cm, the length of the two arms is 10cm, and the angle between the two arms is 90°C. At the ends of the tubes of the two arms, silicone tubes were used to connect the distilled water bottle and the activated carbon bottle in sequence. Finally, the activated carbon bottle was opened in the atmosphere, and the insect release bottle was connected to the suction device with gauze. Cut the filter paper strip into 5 cm × 1 cm, fold it into a "V" shape, and place it in the trap inlet glass tube.
[0050] The Y-shaped olfactometer was tilted 30°, and the temperature was controlled at 25±2°C. Take 100 μl of the attractant of the present invention, formula 1, and formula 2 attractant and ev...
Embodiment 3
[0053] The test site was selected as a greenhouse in the orchard of Minhou, Fujian Province, and the crops were strawberries. Adopt blue sticky insect plate as trapping device, put attractant of the present invention, formula 1, formula 2 respectively in the centrifuge tube, hang on the color plate, the centrifuge tube upper cover is 5 circular holes with a diameter of 0.2mm. The centrifuge tube containing pure liquid paraffin suspended on the blue sticky insect board was used as the control, and the blue sticky insect board suspended with attractant and sprayed with ethyl spinosad was used as the treatment. Three repetitions were set up, and the different treatments were randomly arranged. The swatches are hung 20 cm above the plants, with a span of 6-8 m, and 3 columns are hung in each shed, with a column spacing of 1.5 m and a swatch spacing of 3 m. Investigate the results of 3d, 7d, and 14d hanging insect traps respectively, record the data and replace with new color plate...
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