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Method for using growth model based on salmonella in milk under variable temperature condition

A Salmonella, growth model technology, applied in biochemical equipment and methods, microbial determination/inspection, resistance to vector-borne diseases, etc., can solve problems such as Salmonella growth and growth errors, temperature fluctuations, etc., to achieve the effect of monitoring microbial safety

Pending Publication Date: 2021-01-29
NANJING UNIV OF TECH
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Problems solved by technology

[0005] The existing growth models of Salmonella are generally based on constant temperature and medium growth conditions to predict its growth status. However, in real life, food may not always be under constant temperature conditions, which is accompanied by temperature fluctuations. Salmonella in the medium There is also a certain error in the growth of the growth and the growth in the food

Method used

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  • Method for using growth model based on salmonella in milk under variable temperature condition
  • Method for using growth model based on salmonella in milk under variable temperature condition

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Embodiment 1

[0028] Such as Figure 1-2 As shown, the present invention provides a method for using the method based on the growth model of Salmonella in milk under variable temperature conditions, and the specific steps are as follows:

[0029] Step 1, drawing the growth curve of Salmonella under variable temperature conditions;

[0030] A. Select the growth temperature and growth time, set the growth time period, set the growth temperature at 15°C, 20°C, 25°C, 30°C, 37°C, 47°C, and set the number of growth time periods to 30;

[0031] B. Substitute the time period set in A into the Baranyi and Roberts model and the Cardinal equation model to calculate the number of salmonella bacteria growing in milk;

[0032] C. Calculate the initial concentration of Salmonella in the next time period. If the number of growth bacteria in the previous time period reaches the maximum concentration, the maximum concentration is the initial concentration of Salmonella in the next time period. If the maximu...

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Abstract

The invention discloses a method for using a growth model based on salmonella in milk under a variable temperature condition. The method comprises the following specific steps of step 1, drawing a growth curve of salmonella under the variable temperature condition; and 2, judging whether the milk can be safely eaten or not. According to the method, a growth state of the salmonella in the milk andthe shelf life of the milk can be visually and rapidly predicted, and microbial safety of food can be effectively monitored.

Description

technical field [0001] The invention relates to the field of predictive microbiology, in particular to a method based on a growth model of Salmonella in milk under variable temperature conditions. Background technique [0002] Pathogenic microorganisms account for a large proportion of the causes of various foodborne diseases. Salmonella is one of the most important causes of foodborne illness outbreaks when doses of Salmonella reach 10 5 ~10 9 CFU will cause disease. Among all kinds of food, the media that cause the outbreak of salmonellosis are mainly livestock and poultry meat, eggs and dairy products. [0003] Milk is food that can be eaten immediately, so if the milk is not subjected to strict pasteurization and other disinfection treatments before consumption, then salmonella can easily contaminate the milk, and once consumed by consumers, it is likely to cause serious harm to the body. [0004] According to recent studies, most of the research objects are fresh po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12Q1/06
CPCC12Q1/10C12Q1/06Y02A50/30
Inventor 崔晓文孔佳仪熊晓辉
Owner NANJING UNIV OF TECH
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