Anti-oxidation and anti-glycation composition, application thereof and anti-oxidation and anti-glycation product

Abstract

The invention relates to an anti-oxidation and anti-glycation composition, application thereof and an anti-oxidation and anti-glycation product, and belongs to the technical field of chemistry. Research finds that the composition of ascorbyl glucoside and alpha arbutin has good antioxidant, whitening and moisturizing effects, can inhibit the generation of fluorescent AGEs, has an inhibition rate up to 83.92 + / -0.718%, has a good anti-glycation effect, and has an extremely high application prospect in preparation of anti-oxidation and anti-glycation products.

Description

technical field

[0001] The invention relates to a composition of an oxygen-sugar double antibody and its application and a product of an oxygen-glucose double antibody, belonging to the field of chemical technology. Background technique

[0002] Advanced Glycation End-products (Advanced Glycation End-products, hereinafter referred to as "AGEs") refer to the process of free amino groups of macromolecular substances such as proteins, amino acids, lipids or nucleic acids and aldehyde groups of reducing sugars under non-enzymatic conditions. A group of stable end products produced after condensation, rearrangement, cleavage, and oxidation modification are brownish yellow in color. The process of AGEs production is called protein non-enzymatic glycosylation reaction, which is an irreversible reaction.

[0003] There are two sources of AGEs in the human body, namely endogenous (produced in vivo) and exogenous (dietary intake). Endogenous AGEs are produced by the glycation reacti...

Examples

experiment example 1

[0033] Experimental example 1: Effects of different compounds on the formation of AGEs (BSA-fructose simulation reaction system)

[0034] 1. Preparation of reagents and sample solutions

[0035] Solvent A: Prepare sodium azide with phosphate buffer (50 mmol / L, pH 6.5) to obtain a phosphate buffer containing 0.1% (m / m) sodium azide;

[0036] Aminoguanidine solution: take aminoguanidine and prepare it with solvent A to obtain an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0037] Fructose solution: take fructose and prepare it with solvent A to obtain a fructose solution with a concentration of 1.5mol / L;

[0038] Bovine serum albumin (BSA) solution: take bovine serum albumin and use solvent A to prepare a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0039] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside phosphate buffer (50mmol / L, pH 6.5) to prepare a concentration of 5mg / mL ascorbyl glucoside solution mother solu...

experiment example 2

[0063] Experimental example 2: Effects of different compounds on the formation of AGEs (BSA-MGO simulation reaction system)

[0064] Specific steps are as follows:

[0065] 1. Preparation of reagents and sample solutions

[0066] Solvent A: Prepare sodium azide with phosphate buffer (50 mmol / L, pH 6.5) to obtain a phosphate buffer containing 0.1% (m / m) sodium azide;

[0067] Aminoguanidine solution: take aminoguanidine and prepare it with solvent A to obtain an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0068] Methylglyoxal (MGO) solution: get methylglyoxal and adopt solvent A to prepare the methylglyoxal (MGO) solution that concentration is 60mmol / L;

[0069] Bovine serum albumin (BSA) solution: take bovine serum albumin and use solvent A to prepare a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0070] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside phosphate buffer (50mmol / L, pH 6.5) to prepare a concentrat...

Embodiment 1

[0092] Example 1: Oxyglucose double antibody mask

[0093] The composition of the facial mask liquid that is attached on this facial mask comprises (according to total mass percent is 100%):

[0094] Formula: Ascorbyl Glucoside 20%, Vitamin E 2.0%, Butylene Glycol 5.0%, Sodium Hyaluronate 0.5%, Bisabolol 0.4%, Benzoic Acid 0.1%, and the rest is water.

[0095] Preparation method: Weigh the raw materials according to the above formula and mix them evenly, and prepare the facial mask according to the conventional process in this field.

technical field

[0001] The invention relates to a composition of an oxygen-sugar double antibody and its application and a product of an oxygen-glucose double antibody, belonging to the field of chemical technology. Background technique

[0002] Advanced Glycation End-products (Advanced Glycation End-products, hereinafter referred to as "AGEs") refer to the process of free amino groups of macromolecular substances such as proteins, amino acids, lipids or nucleic acids and aldehyde groups of reducing sugars under non-enzymatic conditions. A group of stable end products produced after condensation, rearrangement, cleavage, and oxidation modification are brownish yellow in color. The process of AGEs production is called protein non-enzymatic glycosylation reaction, which is an irreversible reaction.

[0003] There are two sources of AGEs in the human body, namely endogenous (produced in vivo) and exogenous (dietary intake). Endogenous AGEs are produced by the glycation reacti...

Examples

experiment example 1

[0033] Experimental example 1: Effects of different compounds on the formation of AGEs (BSA-fructose simulation reaction system)

[0034] 1. Preparation of reagents and sample solutions

[0035] Solvent A: Prepare sodium azide with phosphate buffer (50 mmol / L, pH 6.5) to obtain a phosphate buffer containing 0.1% (m / m) sodium azide;

[0036] Aminoguanidine solution: take aminoguanidine and prepare it with solvent A to obtain an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0037] Fructose solution: take fructose and prepare it with solvent A to obtain a fructose solution with a concentration of 1.5mol / L;

[0038] Bovine serum albumin (BSA) solution: take bovine serum albumin and use solvent A to prepare a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0039] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside phosphate buffer (50mmol / L, pH 6.5) to prepare a concentration of 5mg / mL ascorbyl glucoside solution mother solu...

experiment example 2

[0063] Experimental example 2: Effects of different compounds on the formation of AGEs (BSA-MGO simulation reaction system)

[0064] Specific steps are as follows:

[0065] 1. Preparation of reagents and sample solutions

[0066] Solvent A: Prepare sodium azide with phosphate buffer (50 mmol / L, pH 6.5) to obtain a phosphate buffer containing 0.1% (m / m) sodium azide;

[0067] Aminoguanidine solution: take aminoguanidine and prepare it with solvent A to obtain an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0068] Methylglyoxal (MGO) solution: get methylglyoxal and adopt solvent A to prepare the methylglyoxal (MGO) solution that concentration is 60mmol / L;

[0069] Bovine serum albumin (BSA) solution: take bovine serum albumin and use solvent A to prepare a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0070] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside phosphate buffer (50mmol / L, pH 6.5) to prepare a concentrat...

Embodiment 1

[0092] Example 1: Oxyglucose double antibody mask

[0093] The composition of the facial mask liquid that is attached on this facial mask comprises (according to total mass percent is 100%):

[0094] Formula: Ascorbyl Glucoside 20%, Vitamin E 2.0%, Butylene Glycol 5.0%, Sodium Hyaluronate 0.5%, Bisabolol 0.4%, Benzoic Acid 0.1%, and the rest is water.

[0095] Preparation method: Weigh the raw materials according to the above formula and mix them evenly, and prepare the facial mask according to the conventional process in this field.

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