Application of exosome mRNA in breast disease diagnosis

Abstract

The invention relates to the field of biomedical diagnosis, in particular to application of exosome mRNA in breast disease diagnosis. By use of the exosome mRNA, benign and malignant identification can be carried out on breast nodule patients, mammary cancer and other malignant tumors can be effectively distinguished to realize accurate diagnosis of the mammary cancer, and the treatment effect ofpatients can be monitored. It is found for the first time that the exosome mRNA marker can be used for diagnosing breast diseases.

Description

technical field [0001] The present invention relates to the field of biomedical diagnosis, in particular to the application of exosome mRNA in the diagnosis of breast diseases. Background technique [0002] Breast cancer (mammary cancer) is one of the most common malignant tumors in women. The incidence rate accounts for 7-10% of all kinds of malignant tumors in the whole body. It is second only to uterine cancer in women and has become the main cause of threat to women's health. Its incidence is often related to heredity, and between the ages of 40 and 60, the incidence rate of women before and after menopause is higher. It is one of the most common malignant tumors that usually occurs in the glandular epithelial tissue of the breast, seriously affecting women's physical and mental health and even threatening their lives. Breast cancer is rare in men, and only about 1-2% of breast cancer patients are men. [0003] In recent years, the incidence of breast cancer in my coun...

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Problems solved by technology

In addition, the breasts of Chinese women are small and dense, which will also reduce the positive rate of patients receiving mammography

[0007] 3. Early detection of biological targets: including general screening of breast cancer-related genes, such as BRCA1 and BRCA2 mutation analysis, but current molecular diagnosis research mostly focuses on the relationship between gene polymorphisms and breast cancer, which can only give the susceptibility risk of patients, not Applicable to the diagnosis of clinical diseases

In addition, the most commonly used serum tumor markers for breast cancer in clinical practice include CA-153, CEA, CA-125, etc. Although they can reflect the state of the disease to a certain extent, the specificity is not high. In many other tumors, such as ovarian cancer, Pelvic tumors, gastric cancer, colon cancer, liver cancer, etc. will exist accordingly

It is reported that the sensitivity of CA-153 is only about 50%, and the expression level in early breast cancer patients is often low, and the specificity is not high, which has certain limitations in the early screening of breast cancer

[0008] 4. Needle biopsy: The pathological examination of breast tissue needle biopsy has high accuracy, but it is an invasive operation, and false negative results may be produced due to insufficient specimen collection, or due to different sampling sites, intratumoral abnormalities in breast cancer, etc. Qualitative effects can produce different results

[0009] In general: the screening of breast nodules has the following deficiencies: (1) the cost of clinical physical examination is high; (2) the rate of positive and missed detection of imaging diagnostic methods is high; (3) currently more mature biological targets, only Can do risk assessment, not suitable for clinical diagnosis; (4) Puncture biopsy is firstly invasive, and has high requirements for the sampler and puncture site, and it is easy to cause different results due to the influence of external factors

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