A kind of tissue culture rapid propagation method of crabapple

Abstract

The invention discloses a tissue culture rapid propagation method of crabapple, which belongs to the technical field of plant tissue culture. The method comprises the following steps: 1) selecting explants and disinfecting them; 2) treating the explants against browning; 3) inoculating the sterilized explants into the induction medium for axillary bud induction until the axillary buds of the explants are Germinate and grow into plants of 2 to 3 cm; 4) Cut off the plants obtained in step 3) in a sterile environment and inoculate them into the proliferation medium for cultivation, and inoculate the clustered buds that grow out into the proliferation medium for proliferation and growth ; 5) cutting out the clustered buds with a height of 2-3 cm and inoculating them into the rooting medium for rooting culture. The tissue culture of the present invention has simple operation steps, low cost, rapid multiplication to produce a large number of adventitious buds, high-efficiency breeding of a large number of seedlings, and the reproduction offspring retains the excellent traits of the mother plant and has the same traits, shortens the propagation cycle, and is beneficial to the development of new varieties of ornamental crabapple on the market. promote.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for tissue culture and rapid propagation of crabapple. Background technique [0002] Begonia (Malus spp.), also known as Begonia flower, is a deciduous shrub or small tree of Rosaceae (Rosaceae) Malus. . Ornamental crabapple combines flowers, leaves, and fruits. It has rich varieties and a long ornamental period. It has high ornamental value, ecological value and economic value. It has the characteristics of rapid growth and strong adaptability. It is often used as a garden landscape plant. [0003] Begonias have been used in gardens in my country for more than two thousand years. Begonias are often used as the main scene, background scene and other tree species in modern gardens to form a relatively stable and colorful plant community, creating a beautiful garden plant landscape. [0004] Begonia has a certain medicinal value. Begonia fru...

AI Technical Summary

Problems solved by technology

Traditional cuttings and layering have low reproductive coefficients, slow growth, and are greatly affected by climate and other factors. It is difficult to thoroughly disinfect the propagation materials with viruses, and the stable inheritance of excellent traits of crabapple cannot be guaranteed.

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