A kind of tissue culture rapid propagation method of crabapple

A tissue culture rapid propagation and crabapple technology, applied in the field of plant tissue culture, can solve the problems such as the inability to guarantee the stable inheritance of the excellent characteristics of the crabapple, the difficulty of thorough disinfection of virus propagation materials, and the low reproduction coefficient, so as to shorten the breeding cycle and reduce the cost. , the effect of good growth and development

Active Publication Date: 2022-04-01
NANJING FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

Traditional cuttings and layering have low reproductive coefficients, slow growth, and are greatly affected by climate and other factors. It is difficult to thoroughly disinfect the propagation materials with viruses, and the stable inheritance of excellent traits of crabapple cannot be guaranteed.

Method used

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  • A kind of tissue culture rapid propagation method of crabapple

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Embodiment 1

[0026] A method for tissue culture and rapid propagation of a new crabapple variety 'Dream of Orange', specifically as follows:

[0027] 1) Disinfection of explants: cut the young shoots of the current year into 1-2 cm stem segments with 1-2 axillary buds, soak them in 10% detergent (volume percentage) for 30 minutes, rinse them with running water for 2 hours, and place them in Soak in 75% ethanol for 35s in the ultra-clean workbench, rinse with sterile water for 3 to 5 times, and then use 0.1% mercuric chloride (6, 8, 10 min) and 0.5%, 2% sodium hypochlorite (6, 8, 10, 12 min) for treatment, rinsed with sterile water for 6 to 8 times for later use, and cut off the parts of both ends of the stem segment in contact with the disinfection solution for the next step of cultivation.

[0028] The results showed that after disinfection by 0.1% mercuric chloride, the explant survival rate was higher, and the browning rate and pollution rate were also lower. The pollution rate and bro...

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Abstract

The invention discloses a tissue culture rapid propagation method of crabapple, which belongs to the technical field of plant tissue culture. The method comprises the following steps: 1) selecting explants and disinfecting them; 2) treating the explants against browning; 3) inoculating the sterilized explants into the induction medium for axillary bud induction until the axillary buds of the explants are Germinate and grow into plants of 2 to 3 cm; 4) Cut off the plants obtained in step 3) in a sterile environment and inoculate them into the proliferation medium for cultivation, and inoculate the clustered buds that grow out into the proliferation medium for proliferation and growth ; 5) cutting out the clustered buds with a height of 2-3 cm and inoculating them into the rooting medium for rooting culture. The tissue culture of the present invention has simple operation steps, low cost, rapid multiplication to produce a large number of adventitious buds, high-efficiency breeding of a large number of seedlings, and the reproduction offspring retains the excellent traits of the mother plant and has the same traits, shortens the propagation cycle, and is beneficial to the development of new varieties of ornamental crabapple on the market. promote.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for tissue culture and rapid propagation of crabapple. Background technique [0002] Begonia (Malus spp.), also known as Begonia flower, is a deciduous shrub or small tree of Rosaceae (Rosaceae) Malus. . Ornamental crabapple combines flowers, leaves, and fruits. It has rich varieties and a long ornamental period. It has high ornamental value, ecological value and economic value. It has the characteristics of rapid growth and strong adaptability. It is often used as a garden landscape plant. [0003] Begonias have been used in gardens in my country for more than two thousand years. Begonias are often used as the main scene, background scene and other tree species in modern gardens to form a relatively stable and colorful plant community, creating a beautiful garden plant landscape. [0004] Begonia has a certain medicinal value. Begonia fru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李娜云金虎张往祥李广平李利娟孙艳艳楚壮壮
Owner NANJING FORESTRY UNIV
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