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Tissue rapid propagation method of table grapes

A technology for rapid propagation of table grapes and tissue culture, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of no solution for domesticated and transplanted tissue culture seedlings, the toxicity of the disinfectant mercuric acid, the operation Inconvenient and other problems, to reduce the risk of contamination in tissue culture experiments, to facilitate the collection of materials, and to reduce the difficulty of the effect

Inactive Publication Date: 2021-03-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the existing tissue culture and rapid propagation technology of fresh table grapes, new shoots are used as explant materials, and the material is limited by seasons, and the disinfectant mercury liter used to disinfect explants is toxic, and the actual operation is inconvenient
In the proliferation culture, the composition of the medium for differentiation, proliferation and rooting is different, the operation is inconvenient, and there is no complete solution for domestication and transplantation of tissue cultured seedlings, and the induced tissue cultured seedlings cannot be directly applied to field planting

Method used

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  • Tissue rapid propagation method of table grapes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Explant collection

[0057] Collect robust dormant branches of virus-free table grape 'Xiahei', cut the dormant branches into single-bud stem segments, the upper end of the bud is flat, 2-3cm away from the bud, and the lower end is oblique, 3-4cm away from the bud. The physiological dormancy was broken after being treated in a constant temperature water bath at 45°C for 90 minutes, and the single-bud stem segment was floated in clean water with a foam board for cultivation. The culture environment is light intensity 30~40μmol·m -2 ·s -1 (or 2000Lx~3000Lx), the light time is 14~16h, and the temperature is 25±2℃. Sampling was carried out when the length of new shoots reached 10-20 cm, and stem segments cut to 5 cm were used as explant materials.

[0058] (2) Surface disinfection of explants

[0059] Put the stem segment into the container and soak it with detergent for 5-10 minutes, then rinse it with running water for 2-3 hours, and finally rinse it with distille...

Embodiment 2

[0074] Collect the dormant branches of virus-free table grape 'Ruby', treat the dormant branches in hydroponics, disinfect the surface of the explants, inoculate the explants and start the multiplication culture conditions are the same as Example 1. In the proliferation culture, when the medium condition is WPM+IBA0.2mg / L+sucrose 30g / L+agar 6.5-7g / L, the second-generation proliferation coefficient of 'Ruby' is 4.7. At the same time, it germinates and takes root. The root system is developed and the tap root density is relatively high. No browning of the root system; the hydroponic seedling hardening, domestication and transplanting steps are the same as in Example 1, the hydroponic survival rate of Ruby' is 92.0%, and the transplanting survival rate is 92.9%.

Embodiment 3

[0078] Collect the dormant branches of virus-free table grape 'Red Globe', treat the dormant branches in hydroponics, disinfect the surface of the explants, inoculate the explants and start the multiplication culture conditions are the same as Example 1. In the proliferation culture, when the medium condition is WPM+IBA0.2mg / L+sucrose 30g / L+agar 6.5-7g / L, the second-generation multiplication coefficient of 'Red Earth' is 3.3, and at the same time, it germinates and takes root, the root system is well developed and the taproot density is relatively large. There is no browning of leaves and roots; the hydroponic seedling hardening, domestication and transplanting steps are the same as in Example 1, the hydroponic survival rate of 'Red Earth' is 93.3%, and the transplanting survival rate is 96.5%.

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Abstract

The invention relates to the technical field of plant tissue culture seedling raising, in particular to a tissue rapid propagation method of table grapes. The method comprises the following steps of taking dormant branches of table grapes, cutting the dormant branches into single-bud stems, breaking physiological dormancy, carrying out water culture on the single-bud stems until the single-bud stems germinate, and sampling when new shoots are 10-20 cm long to obtain explants; shearing wounds at the top end and the base part of the explant, and inoculating the explant into an initial culture medium for initial culture; when new shoots grow to 5-6 cm, inoculating the new shoots into a propagation culture medium for propagation culture, carrying out subculture once every 40-50 days, and carrying out subculture for 3-4 generations; and selecting tissue culture seedlings for seedling hardening and transplanting. According to the method, the initial culture medium can obviously induce the stem segments of the table grapes to grow new shoots or cluster buds; and the new shoots or cluster buds subjected to initial culture in the propagation culture medium can be subjected to propagation culture and simultaneously germinate and root to form seedlings through one-step induction, and only one propagation culture medium needs to be prepared, so that the propagation efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and seedling cultivation, in particular to a method for rapid tissue propagation of table grapes. Background technique [0002] Fresh table grapes are delicious and nutritious, and high-quality fresh food varieties have high economic value. Utilizing the rapid propagation technology of high-quality table grape varieties can obtain a large number of virus-free seedlings in a short period of time, and the domestication and transplanting technology can make tissue culture seedlings be used for field planting, which is conducive to quickly obtaining high-quality table grape fruits and increasing economic growth. benefit. my country has introduced a variety of high-quality table grape varieties, which are widely planted in many grape producing areas in my country. Fresh table grape 'Xiahei' is an excellent variety. It is a European and American hybrid grape, cultivated in Japan. The skin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 何非朱元娣王军张明悦
Owner CHINA AGRI UNIV
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