Tissue rapid propagation method of table grapes

A technology for rapid propagation of table grapes and tissue culture, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of no solution for domesticated and transplanted tissue culture seedlings, the toxicity of the disinfectant mercuric acid, the operation Inconvenient and other problems, to reduce the risk of contamination in tissue culture experiments, to facilitate the collection of materials, and to reduce the difficulty of the effect
CN112438200AInactive Publication Date: 2021-03-05CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-03-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of plant tissue culture seedling raising, in particular to a tissue rapid propagation method of table grapes. The method comprises the following steps of taking dormant branches of table grapes, cutting the dormant branches into single-bud stems, breaking physiological dormancy, carrying out water culture on the single-bud stems until the single-bud stems germinate, and sampling when new shoots are 10-20 cm long to obtain explants; shearing wounds at the top end and the base part of the explant, and inoculating the explant into an initial culture medium for initial culture; when new shoots grow to 5-6 cm, inoculating the new shoots into a propagation culture medium for propagation culture, carrying out subculture once every 40-50 days, and carrying out subculture for 3-4 generations; and selecting tissue culture seedlings for seedling hardening and transplanting. According to the method, the initial culture medium can obviously induce the stem segments of the table grapes to grow new shoots or cluster buds; and the new shoots or cluster buds subjected to initial culture in the propagation culture medium can be subjected to propagation culture and simultaneously germinate and root to form seedlings through one-step induction, and only one propagation culture medium needs to be prepared, so that the propagation efficiency is improved.
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Description

technical field

[0001] The invention relates to the technical field of plant tissue culture and seedling cultivation, in particular to a method for rapid tissue propagation of table grapes. Background technique

[0002] Fresh table grapes are delicious and nutritious, and high-quality fresh food varieties have high economic value. Utilizing the rapid propagation technology of high-quality table grape varieties can obtain a large number of virus-free seedlings in a short period of time, and the domestication and transplanting technology can make tissue culture seedlings be used for field planting, which is conducive to quickly obtaining high-quality table grape fruits and increasing economic growth. benefit. my country has introduced a variety of high-quality table grape varieties, which are widely planted in many grape producing areas in my country. Fresh table grape 'Xiahei' is an excellent variety. It is a European and American hybrid grape, cultivated in Japan. The skin...

Claims

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