A self-assembled nanomedicine with oxygen-carrying drug and its preparation method with molecular targeting/sonic dynamic therapy
A technology of sonodynamic therapy and nano-medicine, which is applied in the field of biomedicine, can solve problems such as limiting the efficacy of SDT, achieve the effects of reversing drug resistance, enhancing the effect of sonodynamic therapy, and improving the hypoxic microenvironment of tumors
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Embodiment 1
[0045]Disperse 1 g of Cs in 50 mL of DMF, add 2 g of 4-bromophthalic anhydride, stir at 125 °C under nitrogen protection until the solution is clear, pour the hot filtrate into 500 mL of ice water, a white solid precipitates out, and control the temperature for Centrifuge at -8 °C at 5000 r / min for 5 min, and the obtained solid is chitosan substituted with brominated phthalic anhydride. Weigh 1 g of the above product and disperse it in 50 mL of N-methylpyrrolidone, heat it in an oil bath at 50 °C until it is completely dissolved, add 1.66 g of sodium azide to it, and continue the reaction for 24 hours under nitrogen protection. The reaction solution was poured into 100 mL of ethanol to precipitate solids, centrifuged at 12,000 r / min for 5 min, the precipitate was washed with water and acetone, and then vacuum-dried to obtain azide-substituted chitosan. Weigh 1 g of azide-substituted chitosan and disperse it in 30 mL of DMSO, heat and stir for 20-30 min until completely dissolv...
Embodiment 2
[0047] Disperse 10 mg CE in 1 mL DMSO, 1 mg HP was dissolved in 0.25 mL DMSO, 0.25 mL PFOB was dissolved in 0.25 mL chloroform, and the HP solution and the PFOB solution were added dropwise to the CE solution in turn. Stir magnetically for 24 hours, prepare a small beaker with 20 mL of double-distilled water, slowly add the above solution dropwise into double-distilled water under magnetic stirring, stir magnetically for 24 hours at room temperature and avoid light, and place the reaction solution in a place with a molecular weight cut-off of 8000~ 14000 dialysis bags were dialyzed with deionized water for 24 h, and the water was changed every 6 h. The dialysate was dried by a freeze dryer to obtain Er-modified Cs nanoparticles CEPH loaded with PFOB and HP, which were stored at -20°C.
[0048] The prepared CEPH was dispersed to a certain concentration with DMSO, and the UV absorption was measured with a UV spectrophotometer, from figure 1 It can be seen that CEPH contains the...
Embodiment 3
[0050] Take 10 mg CE and disperse in 1 mL DMSO, then add the HP DMSO solution (1 mg HP dissolved in 0.25 mL DMSO) dropwise to the above solution, stir magnetically for 24 h under dark conditions, and prepare 20 mL bis In a small beaker of distilled water, under magnetic stirring, slowly add the above solution dropwise into double distilled water, stir magnetically for 24 hours at room temperature and avoid light, transfer the reaction solution to a dialysis bag with a molecular weight cut-off of 8000-14000, and dialyze in deionized water Treat for 24 h, change the water every 6 h. The dialysate was dried by a freeze dryer to obtain Er-modified Cs nanoparticles (CEH) loaded with HP.
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