A kind of nucleic acid-drug-loaded nanomaterial that increases tumor permeability in response to vegf and its preparation method and application

Abstract

The invention discloses a nucleic acid-loaded nanomaterial that increases tumor permeability in response to VEGF, comprising a drug-loaded nanoparticle core and a nucleic acid shell, and the nucleic acid shell includes DNA 1 、DNA 2 、DNA 3 and DNA 4 , DNA 3 Contains V7T1 nucleic acid aptamer that specifically recognizes VEGF protein. due to DNA 3 Contains VEGF nucleic acid aptamer, which can specifically recognize VEGF protein overexpressed in the tumor microenvironment, making DNA 3 and DNA 4 Shed from nucleic acid-drug-loaded nanoparticles, DNA 2 / DNA 1 The modified albumin drug-loaded nanoparticle has a small particle size and good tumor permeability; and the entire nucleic acid-drug-loaded nanomaterial is mainly composed of nucleic acid and protein, and has good biocompatibility. The invention also discloses the preparation method and application of the nucleic acid-drug-loaded nanometer material, which is simple in operation and low in cost.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a nucleic acid-albumin nanometer drug-loaded material capable of enhancing tumor permeability in response to VEGF, a preparation method and application thereof. Background technique [0002] Targeted drug delivery nanomaterials have been widely used in the treatment of solid tumors to improve the accuracy of cancer diagnosis and the effectiveness of treatment. In general, 50-100nm nanomaterials can prolong blood circulation and effectively gather and stay in tumor tissue through passive targeting (EPR effect). However, the solid tumor microenvironment also includes dense extracellular matrix, high cell packing density, increased interstitial flow pressure, and slow interstitial flow velocity. These factors make it difficult for large-sized nanomaterials to penetrate into deep tumor tissues, thereby significantly reducing the therapeutic effect of solid tumors. On the contrar...

AI Technical Summary

Problems solved by technology

These factors make it difficult for large-sized nanomaterials to penetrate into deep tumor tissues, thereby significantly reducing the therapeutic effect of solid tumors.

On the contrary, small-sized nanomaterials (≤20nm) can diffuse into the deep layer of the tumor, but are easily cleared by the liver and kidney, so they cannot effectively accumulate at the tumor site

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