Excellent tremella sanguinea strain cultivation method
A cultivation method and technology of blood ear, which is applied in the field of cultivation of excellent strains of blood ear, can solve the problems of low crop cultivation yield, less basic research, and low ear yield, so as to increase yield, ensure effectiveness, and improve The effect of ear rate
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[0040] Example 1: A cultivation method of a blood venous bacterium, including the following steps, step one, the obtained parent type, remove the blood ear on the base, after drying the base, first rinse, cut, cut The closest of the original blood ear is groped, and the laboratory is scrolled in 75% of alcohol cotton balls, and then washed with sterile water, and the moisture was suiled with a sterile gauze, and then cut it with a sterile knife. Clean the firewood block of the firewood head, placed in the center of the test tube medium, placed one capsule, cultured at 24-26 ° C, multi-tube comparison screening, selected growth, no murocous pollution The separation, in time to the fresh medium, after multiple selection, the transfer is purified, the fungi grows vigorously, and the late stage is slightly white, the medium is slightly red, that is, the separation gives the mother, and 18- Cultivate 35-40 days at 20 ° C.
[0041] The medium used in the preparation of the mother breed ...
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[0046] Example 2: The method of cultivation of a blood velocity species, and the difference from Example 1 is that the raw material of the soybean cake medium includes 30 parts of soy tanneast, 15 sucrose, 1 part of potassium hydrogen phosphate, 0.5 parts. Seven water combined with magnesium sulfate, 15 servings, water ratio of 1: 1.0-1.2 (weight ratio). The raw materials of the second-generation mother culture include 60 parts of sawdust, 20 serviles, 1 part of gypsum powder, 1 gypsum powder, 0.5 pieces of sugar, the water ratio of 1: 1.0-1.2. In step four, the concentration of the sugar was 0.05%.
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[0047] Example 3: Taking the cultivation method of hemathered bacteria, the difference from Example 1 is: Yellow bean cake medium raw material comprises 30 parts of soybean cake powder, 15 sucrose, 2 parts of potassium hydrogen phosphate, 1 part Seven water combined with magnesium sulfate, 15 servings, water ratio of 1: 1.0-1.2 (weight ratio). The raw materials of the second-generation mysterian medium include: 75 serviles, 30 parts of wheat bran, 2.5 plaster powder, 2.5 pieces of plaster powder, 2 sugar, the water ratio is 1: 1.0-1.2 . In step four, the concentration of the sugar is 2%.
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