Excellent tremella sanguinea strain cultivation method
A cultivation method and technology of blood ear, which is applied in the field of cultivation of excellent strains of blood ear, can solve the problems of low crop cultivation yield, less basic research, and low ear yield, so as to increase yield, ensure effectiveness, and improve The effect of ear rate
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Embodiment 1
[0040] Embodiment 1: a kind of cultivation method of blood ear excellent bacterial strain, comprises the following steps, step 1, the acquisition of mother species, removes the blood ear on the base wood, after the base wood is dried, rinses clean repeatedly with running water earlier, cuts out The base wood of the part closest to the growth of the original blood ear was scrubbed several times with 75% alcohol cotton balls in the laboratory, then washed with sterile water, dried with sterile gauze, and cut open with a sterile knife. Clamp the base wood block the size of a matchhead, place it in the center of the prepared test tube culture medium, put one grain in each tube, and cultivate it at 24-26°C. After comparing and screening multiple tubes, select the one that grows vigorously and is free from bacterial contamination. The isolates were transferred to fresh culture medium in time, after multiple selections, transferred to tubes for purification, the mycelium grew vigorous...
Embodiment 2
[0046] Embodiment 2: A kind of cultivation method of blood ear fine strain, the difference with embodiment 1 is that the raw material of soybean cake culture medium comprises 30 parts of soybean cake powder, 15 parts of sucrose, 1 part of dipotassium hydrogen phosphate, 0.5 part of Magnesium sulfate heptahydrate, 15 parts of agar, the ratio of material to water is 1:1.0-1.2 (weight ratio). In parts by weight, the raw materials of the second-generation mother seed medium include 60 parts of sawdust, 20 parts of wheat bran, 1 part of gypsum powder, 1 part of gypsum powder, and 0.5 part of sugar, and the ratio of material to water is 1:1.0-1.2. In step 4, the concentration of sugar water is 0.05%.
Embodiment 3
[0047] Embodiment 3: A kind of breeding method of excellent bacterial strain of blood ear, the difference with embodiment 1 is: the raw material of soybean cake culture medium comprises 30 parts of soybean cake powder, 15 parts of sucrose, 2 parts of dipotassium hydrogen phosphate, 1 part of Magnesium sulfate heptahydrate, 15 parts of agar, the ratio of material to water is 1:1.0-1.2 (weight ratio). In terms of parts by weight, the raw materials of the second-generation mother seed medium include: 75 parts of sawdust, 30 parts of wheat bran, 2.5 parts of gypsum powder, 2.5 parts of gypsum powder, 2 parts of sugar, and the ratio of material to water is 1:1.0-1.2 . In step 4, the concentration of sugar water is 2%.
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