Insecticidal compound microbial agent as well as preparation method and application thereof

A compound microbial inoculum, Lactobacillus plantarum technology, applied in microorganism-based methods, biochemical equipment and methods, biocides, etc., can solve problems such as easy generation of drug resistance and environmental pollution

Inactive Publication Date: 2021-03-16
中诚国联(河南)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, chemical pesticides are mainly used in the prevention and control of crop diseases and insect pests, which are not only easy to produce drug resistance, but also cause serious environmental pollution and other problems, thus requiring compound microbial agents that can kill insects

Method used

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  • Insecticidal compound microbial agent as well as preparation method and application thereof
  • Insecticidal compound microbial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare the following raw materials by weight according to the proportions: 10 parts of Metarhizium anisopliae, 10 parts of Bacillus thuringiensis, 6 parts of mixed fermentation broth of Bacillus subtilis, 8 parts of mixed fermentation broth of Bacillus licheniformis, Lactobacillus plantarum, humic acid 1 part, 10 parts of subcritical hydrolysate, 4 parts of dispersion medium, 1 part of antioxidant, 1 part of stabilizer; respectively take two rings of Metarhizium anisopliae and Bacillus thuringiensis, and inoculate them in beef extract peptone medium respectively, and the culture temperature is 30 ℃ , the culture time was 20h, and the seed liquids of Metarhizium anisopliae and Bacillus thuringiensis were obtained respectively; the seed liquid was inserted into 500L seed tank medium according to the inoculum of 5%, and cultivated at 30°C for 24h to obtain Metarhizium anisopliae and Bacillus thuringiensis respectively. Liquid; Mixed fermentation liquid of Metarhizium aniso...

Embodiment 2

[0035]Prepare the following raw materials by weight according to the proportions: 12 parts of Metarhizium anisopliae, 12 parts of Bacillus thuringiensis, 7 parts of Bacillus subtilis-Bacillus ayebo mixed fermentation broth, 9 parts of Bacillus licheniformis-Lactobacillus plantarum mixed fermentation broth, rot 1.5 parts of polygenic acid, 13 parts of subcritical hydrolysate, 6 parts of dispersion medium, 1.2 parts of antioxidant, and 1.3 parts of stabilizer; respectively, two rings of Metarhizium anisopliae and Bacillus thuringiensis were respectively inoculated into beef extract peptone medium, and the culture temperature was 32 ℃, culture time 20h, get the seed liquid of Metarhizium anisopliae and Bacillus thuringiensis respectively; insert the seed liquid into 500L seed tank medium according to 5% inoculum, and cultivate at 302 ℃ for 26h to get Metarhizium anisopliae and Bacillus thuringiensis respectively. Bacillus strain; Metarhizium anisopliae, Bacillus thuringiensis stra...

Embodiment 3

[0045] Prepare the following raw materials by weight according to the proportions: 15 parts of Metarhizium anisopliae, 15 parts of Bacillus thuringiensis, 8 parts of Bacillus subtilis-Bacillus Ayebo mixed fermentation broth, 11 parts of Bacillus licheniformis-Lactobacillus plantarum mixed fermentation broth, rot 2 parts of reproductive acid, 15 parts of subcritical hydrolysate, 7 parts of dispersion medium, 1.5 parts of antioxidant, and 1.5 parts of stabilizer; respectively take two rings of Metarhizium anisopliae and Bacillus thuringiensis, and inoculate them in beef extract peptone medium respectively. 35 ℃, culture time 20h, get the seed liquid of Metarhizium anisopliae and Bacillus thuringiensis respectively; insert the seed liquid into 500L seed tank medium according to 5% inoculum, and cultivate at 35 ℃ for 27h to get Metarhizium anisopliae and Bacillus thuringiensis respectively. Bacillus strain; Metarhizium anisopliae, Bacillus thuringiensis strain, Bacillus subtilis-Ba...

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Abstract

The invention discloses an insecticidal compound microbial agent, which is prepared from the following raw materials: green muscardine fungus, bacillus thuringiensis, bacillus subtilis and bacillus aryabhattai mixed fermentation liquor, bacillus licheniformis and lactobacillus plantarum mixed fermentation liquor, humic acid, sub-critical hydrolysate, a dispersion medium, an antioxidant and a stabilizer. The invention further discloses a preparation method of the insecticidal compound microbial agent, green muscardine fungi and bacillus thuringiensis are added, through synergistic interaction of the green muscardine fungi and the bacillus thuringiensis, the quite outstanding prevention and treatment effect is achieved, by adding thebacillus subtilis and bacillus aryabhattai mixed fermentation liquor and thebacillus licheniformis and lactobacillus plantarum mixed fermentation liquor, the synergistic growth and metabolism among strains are further facilitated, synergistic effect is achieved for killing insects, and the insect killing effect is improved. Humic acid is used as a protective agent, and sub-critical hydrolysate is used for providing nutrients, so that the germination rateand the survival rate of microbial thalli can be effectively improved, and a certain guarantee is provided for long-acting insect killing of microorganisms.

Description

technical field [0001] The invention relates to the field of microbial inoculants, in particular to an insecticidal compound microbial inoculum and a preparation method and application thereof. Background technique [0002] Microbial inoculum refers to a live bacterial preparation processed from the fermentation broth of target microorganisms (effective bacteria) after industrial production and propagation, using porous substances as adsorbents (such as peat, vermiculite) to adsorb bacterial cells. This inoculant is used for seed dressing or root dipping, and has the functions of directly or indirectly improving soil, restoring soil fertility, preventing soil-borne diseases, maintaining the balance of rhizosphere microflora, and degrading toxic substances. The proper use of agricultural microbial inoculants can increase the yield of agricultural products, improve the quality of agricultural products, reduce the amount of chemical fertilizers, reduce costs, improve soil, and ...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N63/23A01N63/22A01N63/20A01N25/22A01P7/00C12N1/20C12N1/14C12R1/645C12R1/125C12R1/07C12R1/10C12R1/25
CPCA01N25/22A01N63/20A01N63/22A01N63/23A01N63/30C12N1/14C12N1/20
Inventor 祝春华朱永杰张君怡李献
Owner 中诚国联(河南)生物科技有限公司
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