Mesenchymal stem cell-based fusion cancer cell membrane bionic nanoparticle and preparation method thereof

A biomimetic nanotechnology, cancer cell membrane technology, applied in non-active ingredients medical preparations, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as difficult to achieve cure, achieve good broad-spectrum and adjustability , good safety and clinical application prospects, good biocompatibility and safety effects

Pending Publication Date: 2021-03-19
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although bone-protective agents currently used to treat bone metastases, such as bisphosphonates and radium 233, can delay the progression of bone metastases, they are still difficult to achieve the goal of cure

Method used

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  • Mesenchymal stem cell-based fusion cancer cell membrane bionic nanoparticle and preparation method thereof
  • Mesenchymal stem cell-based fusion cancer cell membrane bionic nanoparticle and preparation method thereof
  • Mesenchymal stem cell-based fusion cancer cell membrane bionic nanoparticle and preparation method thereof

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Experimental program
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Embodiment 1

[0043] Embodiment 1 prepares each nanoparticle inner core:

[0044] (1) Preparation of lipoic acid nanoparticles LA-NP: 100mg lipoic acid (LA) and 17mg cysteine ​​(Cys) were dissolved in 1mL methanol, stirred at room temperature for 8h. Dry the methanol with nitrogen, add 1mL of dichloromethane to dissolve, and add 4mL of 1% sodium cholate solution at the same time, 400W, ultrasonic emulsification for 10s, repeat 2 times. Carefully add 10mL of double-distilled water with stirring, stir for 8h, centrifuge at 3000rpm for 10min, then continue to centrifuge at 3500rpm for 10min with a 100kMDW ultrafiltration tube to obtain purified LA-NP;

[0045] (2) Preparation of LACLss micelles: LACL polypeptide (LA-KVRVRVRVpPTRVRERVK-NH2, p is D-proline) was synthesized by solid-phase synthesis, LACL and excess cysteine ​​were stirred and cross-linked in methanol for 12 hours, nitrogen Blow dry methanol, dissolve LACLss in double distilled water, self-assemble to form LACLss micelles;

[00...

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Abstract

The invention belongs to the technical field of medicinal preparations, and relates to a mesenchymal stem cell-based fusion cancer cell membrane bionic nanoparticle, in particular to a mesenchymal stem cell-based tumor and bone metastatic tumor targeted fusion cancer cell membrane bionic nanoparticle (TB@NP) and a preparation method and application thereof. The bionic nanoparticle is a drug-loadednanoparticle, and is coated with a layer of fusion cell membrane; the fusion membrane consists of a mesenchymal stem cell membrane and a cancer cell membrane, and is made into a bionic nano drug delivery system capable of targeting tumors and tumor bone metastatic parts. The bionic nanoparticle can be an inorganic nanoparticle and organic nanoparticle, and entrapped drugs can be gene drugs, smallmolecule drugs and the like. In-vivo and in-vitro experiments prove that the bionic nano delivery system can accurately deliver drugs to tumors and tumor bone metastatic parts, is high in targeting property and high in safety, can enhance the curative effect of the drugs and reduce toxic and side effects, and has relatively high clinical practice significance.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and specifically relates to a bionic nanoparticle (abbreviated as TB@NP) based on mesenchymal stem cells fused with cancer cell membranes targeting tumors and bone metastatic tumors, and its preparation method and application. Background technique [0002] The prior art discloses that tumor is a disease that seriously threatens the survival and health of people all over the world. Relevant statistics show that the annual morbidity and mortality of the disease remain high, second only to cardiovascular and cerebrovascular diseases. Studies have shown that in the progress of tumors, most of them will metastasize, and bone is a good site for tumor metastasis. Among them, prostate cancer, breast cancer, ovarian cancer, kidney cancer, lung cancer, liver cancer, cervical cancer, rectal cancer, etc. All cancers are prone to bone metastasis; especially prostate cancer and breast cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K47/20A61K47/18A61K47/42A61K47/34A61K47/04A61K31/337A61P35/00
CPCA61K9/5176A61K47/20A61K47/183A61K47/42A61K47/34A61K47/02A61K31/337A61P35/00
Inventor 高原陈继源
Owner FUDAN UNIV
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