Application of Tripterygium triterpenic acid in preparation of medicine for preventing and treating leukoplakia syndrome virus

A technology of vitiligo syndrome and tripterygium twig, which is applied in the application field of tripterygium triterpene acid in the preparation of medicines for the prevention and treatment of vitiligo syndrome virus, can solve the problems such as the unclear physiological activity function and application range of tripterygium triterpene acid, and achieve prevention Effects of infection and spread

Active Publication Date: 2021-03-19
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In general, the specific physiological activity, function and scope of application of Tripterygium wilfordii triterpenic acid are still unclear, and there are no reports about using tripterygium triterpenic acid to prepare drugs for the prevention and treatment of shrimp white spot syndrome, especially oral drugs

Method used

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  • Application of Tripterygium triterpenic acid in preparation of medicine for preventing and treating leukoplakia syndrome virus
  • Application of Tripterygium triterpenic acid in preparation of medicine for preventing and treating leukoplakia syndrome virus
  • Application of Tripterygium triterpenic acid in preparation of medicine for preventing and treating leukoplakia syndrome virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The in vivo experimental protocol is shown in the table below.

[0030]

[0031] (1) WSSV-free Procambarus clarkii: 120 WSSV-free Procambarus clarkii (20-30 g each) were selected for the challenge experiment. Ensure that each Procambarus clarkii has the same body shape, health status and vitality.

[0032] WSSV-TW suspension, copy number 8 × 10 7 copies / μL, purified by our laboratory.

[0033] (2), the experimental process:

[0034] a. Randomly distribute healthy and active WSSV-free Procambarus clarkii into each shrimp tank with 10 in each group and mark the groups.

[0035] b. Dilute 400 μL (concentration of 1 mg / mL) of the mother liquor of tripterygium terpenoides to obtain 4 mL of medicinal liquid with a concentration of 100 μg / mL, and then dilute it in a 10-fold gradient to prepare a four-part preparation of the tripterygium triterpenoids of the drug drug solution with a concentration gradient. The four concentrations are 0.1μg / mL, 1μg / mL, 10μg / mL, 100μg / mL...

Embodiment 2

[0051] Since Procambarus clarkii has a certain resistance to WSSV, and Penaeus vannamei has almost no resistance to WSSV, in order to further verify that tripterygium triterpenic acid (CAS: 86632-20-4) can be used in different genera and species of shrimp It still has a similar protective effect. On the basis of Example 1, the further verification and optimization of the experiment was carried out, and Penaeus vannamei was selected as the experimental shrimp species, and the oral challenge experiment was carried out by drug-encapsulated WSSV.

[0052] The in vivo experimental protocol is shown in the table below.

[0053]

[0054] (1) WSSV-free Penaeus vannamei: 50 WSSV-free Penaeus vannamei (weight: 10.5-12 g, length: 10.5-13.5 cm) were selected for the challenge experiment. Ensure that each vannamei is consistent in size, health and vitality.

[0055] WSSV-TW suspension, copy number 8 × 10 7 copies / μL, purified by our laboratory.

[0056] (2), the experimental process:...

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Abstract

The invention belongs to the field of disease prevention and treatment of aquaculture, and relates to application of Tripterygium triterpenic acid in preparation of a medicine for preventing and treating leukoplakia syndrome virus, and particularly relates to the application in the preparation of medicines for preventing and treating shrimp white spot syndrome virus. The Tripterygium triterpenic acid is applied in an oral feeding or oral injection mode. Experiments prove that the protection rate of Tripterygium triterpenic acid on procambarus clarkia and penaeus vannamei boone can reach 90% orabove. Tripterygium triterpenic acid can be used for preventing WSSV from infecting a host in an oral mode, can effectively prevent infection and transmission of WSSV as a medicine for preventing andtreating the white spot syndrome of shrimps, and becomes a specific medicine for preventing WSSV infection in the shrimp breeding industry.

Description

technical field [0001] The invention belongs to the field of disease prevention and treatment of aquaculture, and particularly relates to the application of tripterygium triterpene acid in the preparation of medicines for preventing and treating white spot syndrome virus. Background technique [0002] White spot disease (White Spot Disease) broke out in the early 1990s and is an acute, high-mortality disease caused by white spot syndrome virus (WSSV) in shrimp. Once the prawns are infected, 100% of them will die within 3-7 days. WSSV is a large rod-shaped double-stranded circular DNA virus with a genome size of about 300 kbp and is classified in the Whispovirus genus. WSSV has a wide range of crustacean hosts, including farmed or wild prawns, crabs, lobsters, freshwater cultured Macrobrachium rosenbergii, etc. WSSV is lethal to all shrimp species, although susceptibility varies between different hosts. The clinical symptoms of shrimp infected with WSSV include lethargy, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61K9/08A61P31/20
CPCA61K31/58A61K9/0095A61P31/20
Inventor 王永杰李凯夏文旭江录志刘昱辰覃冰
Owner SHANGHAI OCEAN UNIV
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