Compound plant disinfectant as well as preparation method and application thereof

A disinfectant and plant technology, applied in the fields of botanical equipment and methods, disinfectants, plant growth regulators, etc., can solve the problems of easy crystallization, narrow antibacterial spectrum, instability, etc., and achieve the effect of fast action and widening application surface.

Pending Publication Date: 2021-04-02
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single plant disinfection product generally has defects such as high research and development costs, narrow antibacterial spectrum, and easy to make bacteria resistant to antibiotics. If two plant disinfection products are mixed, it is unstable, easy to crystallize, poor storage performance, and not very good bactericidal effect.

Method used

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  • Compound plant disinfectant as well as preparation method and application thereof
  • Compound plant disinfectant as well as preparation method and application thereof
  • Compound plant disinfectant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Sophocarpine, chlorogenic acid, and honeysuckle extract were formulated according to the ratio of 5:4:1, and the total mass of 20-30 mg was mixed with 2-3 mg of 3% Tween-80 aqueous solution, stirred in a constant temperature water bath at 60°C for 30 minutes, dripped Add 1-2 drops of 1-3% triclosan ethanol solution, add 1.5 mg of phosphate-buffered saline (PBS) with a pH of 8.0, and add PBS To about 90mL, after stirring and dissolving at 150 rpm for 1h, the above solution was distilled to 100mL with the same PBS to prepare a new compound plant disinfectant.

[0024] Wherein, when 3% Tween-80 aqueous solution is prepared, according to the solution formed after the Tween-80 of 3mL is mixed with 97mL water.

[0025] The 1-3% triclosan ethanol solution is obtained by mixing 1-3 g of triclosan into 100 mL of absolute ethanol.

[0026] The preparation method of Sophora flavescens extract is to weigh 8-12g of Sophora flavescens powder, add 100ml of 65% ethanol to soak for 24 ...

Embodiment 2

[0028] Sophocarpine, chlorogenic acid, and honeysuckle extract were formulated at a ratio of 5:4:1. Mix 25 mg of the total mass with 2.5 mg of 3% Tween-80 aqueous solution, stir in a constant temperature water bath at 60°C for 30 minutes, and drop 2 drops of 1.5% ethanol solution of triclosan, add 1.5 mg of phosphate buffered saline (PBS) with a mass fraction of 0.7% Sophora flavescens extract and a pH of 8.0, and add PBS to about 90 mL, at 150 rpm After stirring and dissolving for 1 h, the above solution was dilute to 100 mL with the same PBS to prepare a new compound plant disinfectant.

[0029] The preparation method of Sophora flavescens extract is to weigh 10 g of Sophora flavescens powder, add 100 mL of 65% ethanol to soak for 24 hours, and then diafilter. 8.0 phosphate-buffered saline (PBS) to prepare a mass fraction of 0.7% Sophora flavescens extract.

[0030] The same as the above steps, except that sophocarpine-chlorogenic acid-honeysuckle extract was not added to p...

Embodiment 3

[0043] Adopt the accelerated test method, the compound plant disinfectant stock solution configured by the method of Example 1 is sealed and packaged, placed in a 54°C incubator, and stored for 14 days, and its color, clarity change, and test are observed before and after storage respectively. pH, by quantitative bactericidal test in embodiment 2, detect its killing effect, to evaluate the stable performance of this novel compound plant disinfectant. The results are shown in Table 3.

[0044] Table 3 compound plant disinfectant stability test result

[0045]

[0046] The results showed that after storage at 54°C for 14 days, the new compound plant disinfectant did not change significantly, the pH was neutral, and it was a deep red clear solution without insoluble matter. The killing effect on Escherichia coli, Staphylococcus aureus and Candida albicans for 1 minute is 100% before storage and 99.99% after storage. Before and after storage, there is no significant differenc...

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Abstract

The invention relates to a novel compound plant disinfectant. The novel compound plant disinfectant comprises the following components in parts by weight of 4-6 parts of sophocarpine, 3.2-4.8 parts ofchlorogenic acid, 0.8-1.2 parts of a honeysuckle extract, 8-12 parts of an aqueous solution containing Tween-80 and 0.6-0.9 part of PBS dissolved with a sophora flavescens extracting solution, wherein when the unit of previous parts by mass is mg, and the PBS solution with pH value of 8.0 is added to 100 mL. According to the plant disinfectant provided by the invention, the characteristics of complexity of natural effective components, difficulty in causing resistance of thalli and quick action of chemical sterilization substances are fully utilized, the antibacterial spectrum of the plant disinfectant on escherichia coli, staphylococcus aureus and candida albicans is effectively enhanced, the application range is widened, the plant disinfectant has good stability, and the requirements onmultiple aspects of antibiosis, storage and the like can be met.

Description

technical field [0001] The invention belongs to the technical field of disinfectants, and in particular relates to a compound plant disinfectant, its preparation method and application. Background technique [0002] Escherichia coli, Staphylococcus aureus, and Candida albicans are common pathogenic fungi. In the presence of various susceptible factors such as immunosuppression, organ transplantation, and acquired immunodeficiency syndrome, invasive pathogenic fungi will It seriously threatens the patient's safety and needs to be treated with corresponding antibiotics or antibiotics. At present, one of the main technical means to effectively control its infection and spread is daily disinfection. With the progress of society, people's requirements for quality of life and awareness of environmental protection are increasing day by day, and disinfection products that are green, easy to remove, safe to use, non-toxic and tasteless have become people's first choice. Therefore, ...

Claims

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Application Information

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IPC IPC(8): A61K36/355A61K36/489A61K9/08A61K47/26A01N65/20A01N65/08A01N43/90A01N37/38A01N31/16A01N25/30A01P1/00A01P3/00A61P31/02A61P31/04A61P31/10A61K31/216A61K31/4375A61K31/085
CPCA01N25/30A01N31/16A01N37/38A01N43/90A01N65/00A01N65/08A01N65/20A61K9/0014A61K9/08A61K31/085A61K31/216A61K31/4375A61K36/355A61K36/489A61K47/26A61P31/02A61P31/04A61P31/10A61K2300/00Y02A50/30
Inventor 慈颖张晓龙杨燕王思于鹰畅张珊
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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