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A kind of plant fertility-related protein and its application

A plant and protein technology, applied in the field of plant fertility-related proteins and their applications, can solve the problems of limiting the mining and utilization of soybean germplasm resources, limiting the aggregation and utilization of excellent gene loci, etc.

Active Publication Date: 2022-03-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This severely limits the excavation and utilization of soybean germplasm resources, and severely limits the aggregation and utilization of excellent gene loci

Method used

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  • A kind of plant fertility-related protein and its application
  • A kind of plant fertility-related protein and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the acquisition of soybean male sterility gene GmMS1

[0065] The soybean genome was sequenced and functionally analyzed, and a soybean male sterility gene was found, which was named GmMS1 gene. Its CDS is shown in sequence 2 of the sequence listing, and its genome sequence is shown in sequence 7 of the sequence listing. The protein encoded by the GmMS1 gene is named GmMS1 protein, as shown in sequence 1 of the sequence listing.

Embodiment 2

[0066] Example 2, Application of GmMS1 Gene in Regulating Soybean Fertility

[0067] 1. Construction of Crisper / CAS9 gene editing vector

[0068] 1. Design and synthesis of gRNA targets

[0069] Design two target sequences, respectively

[0070] Target 1: GACGGGAACACCTGTGGCGG TGG ;

[0071] Target 2: CGCCGAGGTCTAAGATACAG AGG .

[0072] In target 1 and target 2, the underline is the PAM sequence.

[0073] Two pairs of primers were designed according to the target sequence:

[0074] Target 1 primer:

[0075] F1:5'-TTGGACGGGAACACCTGTGGCGG-3';

[0076] R1: 5'-AACCCGCCACAGGTGTTCCCGTC-3'.

[0077] Target 2 primers:

[0078] F2: 5'-TTGCGCCGAGGTCTAAGATACAG-3';

[0079] R2: 5'-AACCTGTATCTTAGACCTCGGCG-3'.

[0080] 2. Formation of primer dimers

[0081] Dilute primer F1 and primer R1 to 10 μM respectively, and configure the reaction system: F15 μl, R15 μl, H 2 O 15 μl. After mixing, react at 95°C for 3 minutes, then naturally cool to 25°C, and then 16°C for 5 minutes to ...

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Abstract

The invention discloses a plant fertility-related protein and its application. The invention protects the method for obtaining male sterile plants by reducing or inhibiting the activity and / or content of GmMS1 protein in the target plants. The invention also protects the method for obtaining male sterile plants by silencing or inhibiting the expression of GmMS1 gene in the target plant or knocking out the GmMS1 gene. The invention has great significance for the research of plant fertility and the breeding of sterile plants.

Description

technical field [0001] The invention relates to the field of soybean molecular genetic breeding, in particular to a protein related to plant fertility and its application. Background technique [0002] Soybean is the most important oil crop and high-protein food crop in the world. In my country, soybean is one of the four major food crops, which plays a very important role in ensuring national food security, improving urban and rural people's lives and increasing farmers' income. [0003] Soybean is a typical short-day crop. The suitable planting range of a single variety is mostly between 1-1.5 latitudes. The introduction of soybeans between different latitudes will lead to early or delayed flowering time and maturity due to changes in the length of sunshine, resulting in decreased yield and even grains. No income. At the same time, my country has a large geographical area, many ecological types, and unbalanced breeding levels. It is urgent to strengthen the exchange and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29C07K14/415A01H5/00A01H6/54
CPCC07K14/415C12N15/8218C12N15/8289C12N15/8213C12N9/22C12N15/8287A01H1/02C12N2310/20C12N15/111
Inventor 蒋炳军孙石陈莉韩天富岳岩磊侯文胜刘路平袁珊武婷婷
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI