Method for the diagnosis of amyloid-associated diseases

A technology for amyloidosis, amyloidosis, applied in the field of time-dependent growth, active agents for the treatment of amyloid-related diseases, related uses and kits, which can solve the problems of delaying timely and appropriate treatment time, health hazards, etc.

Pending Publication Date: 2021-04-09
阿米罗伊迪亚瑞典股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This significantly delays the time until patients can receive t

Method used

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  • Method for the diagnosis of amyloid-associated diseases
  • Method for the diagnosis of amyloid-associated diseases
  • Method for the diagnosis of amyloid-associated diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0422] Example 1: Analysis in serum

[0423] overview

[0424] By using time-resolved detection of Thioflavin T (ThT) fluorescence intensity fluctuations in sub-femtoliter sized viewing volume elements, we were able to detect apparently healthy individuals and diagnoses with Alzheimer's with single-molecule sensitivity Very low levels of small structured amyloid nanoplaques in the serum of patients with AD. The frequency and size of small structured amyloid nanoplaques encountered in the serum of individuals diagnosed with AD was significantly higher than in controls. Our results demonstrate that, unlike bulk fluorescent ThT spectroscopy, time-resolved ThT fluorescence intensity fluctuation analysis can directly detect, quantify and measure the size of small structured amyloid nanoplaques in serum. The method relies neither on the use of immune-based probes nor on the use of radiotracers, signal amplification, or protein separation techniques, and provides a minimally ...

Embodiment 2

[0505] Example 2: Analysis in CSF

[0506] A 0.5 ml aliquot of cerebrospinal fluid (CSF) stored in polypropylene tubes at -80°C without any additives was used. The samples were allowed to thaw at room temperature for 60 min. Thereafter, 200 μl aliquots were transferred to 8-well chamber coverslips containing 10 μl ThT aqueous solution in each well. Samples were gently mixed by partitioning / extracting several times and analyzed. Analysis was performed in duplicate.

Embodiment 3

[0507] Example 3: Analysis in urine

[0508] First morning urine midstream specimens from apparently healthy donors were collected into a sterile container without any urine preservative. Allow the samples to settle for 60 min at room temperature. Thereafter, 200 μl aliquots were transferred to 8-well chamber coverslips containing 10 μl ThT aqueous solution in each well. Samples were gently mixed by partitioning / extracting several times and analyzed. Analysis was performed in duplicate.

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Abstract

The invention relates generally to methods for the diagnosis and/or prognosis of an amyloid-associated disease, methods of treating an amyloid-associated disease, and methods of identifying an agent for the treatment of an amyloid-associated disease, as well as related uses and kits of parts.

Description

Background technique [0001] The present invention generally relates to methods for diagnosing and / or prognosing amyloid-related diseases, methods for treating amyloid-related diseases and methods for identifying active agents for treating amyloid-related diseases, and related Uses and kits. [0002] Amyloid-related diseases are characterized by the presence and accumulation and aggregation of amyloid fibrils in patients. Well-known examples of amyloid-related diseases are Alzheimer's disease, Huntington's disease and Parkinson's disease. These diseases often lead to deterioration of the patient's health and psychological status, sometimes for a long time and often to death. The pathogenesis and symptoms of amyloid-related diseases are often very traumatic for patients and their families. Alzheimer's disease is the most common cause of dementia worldwide (Prince et al., World Alzheimer Report 2016. London, UK: 2016 and Alzheimer's Disease Facts and Figures. Alzheimer's Assoc...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/68
CPCG01N33/5091G01N33/6896G01N21/6428G01N2021/6417G01N2021/6439G01N2800/2821
Inventor V·弗克杰维奇A·格拉斯伦德J·扎威特A·缇曼R·里格勒尔L·特雷纽斯S·沃姆兰德
Owner 阿米罗伊迪亚瑞典股份公司
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