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Application of metabolites of amylase Streptomyces chromogenes in the control of diseases of Dendrobium officinale

A technology of Streptomyces chromogenes and metabolites, applied in the field of microorganisms, can solve problems such as insecurity, and achieve the effects of avoiding long-term application, good environmental compatibility, and efficient control methods

Active Publication Date: 2021-08-17
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to address the shortcomings of unsafe chemical pesticides and fewer types of microbial source pesticides, and to provide an application of a biocontrol strain of amylase chromogenic Streptomyces diastatochromogenes metabolites in the prevention and treatment of Dendrobium officinale diseases

Method used

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  • Application of metabolites of amylase Streptomyces chromogenes in the control of diseases of Dendrobium officinale
  • Application of metabolites of amylase Streptomyces chromogenes in the control of diseases of Dendrobium officinale

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: (the liquid fermentation of amylase Streptomyces chromogenicum tz3-1)

[0021] Streptomyces diastatochromogenes tz3-1 was obtained from Streptomyces diastatochromogenes D through ribosome engineering. For details, please refer to the applied invention patent (application number: 2019114179541).

[0022] Fermentation broth contains 20g of soluble starch per 1000mL, CaCO 3 5g, KH 2 PO4 3g, NH 4 Cl 3g, FeCl 2 1g, 43g soybean powder, and water as the balance; 60mL fermentation broth per 300mL triangular bottle, after preparation, sterilize at 121°C for 20min, cool to 50°C, pick a platinum cycloamylase chromogenic Streptomyces D under sterile conditions Or the corresponding mutant strain inoculation, 28 ℃ ± 1 ℃, fermentation culture for 5 days; the fermentation broth was centrifuged at 5000r / min for 10min, and the supernatant was filtered through a 0.22μm membrane and used for antibacterial activity determination and field control effect test.

Embodiment 2

[0023] Embodiment 2: (Amylase Streptomyces chromogenes tz3-1 metabolite antibacterial activity assay)

[0024] Take 1mL of the fermentation supernatant filtered through a 0.22μm membrane in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C. After cooling, place a test bacteria with a diameter of 5mm on the medium plane The fungus cake, the fungus cake is connected to the center of the petri dish (the diameter of the petri dish is 9cm), placed in an incubator for 36 hours at 28°C, and treated with sterile water as a control, repeated 3 times; the diameter of the colony is measured by the cross method, and the following formula is used: Calculation of inhibition rate: Mycelia growth inhibition rate (%)=[(control colony diameter-5)-(treatment colony diameter-5)] / (control colony diameter-5)×100%.

[0025] The results showed that the metabolites of amylase Streptomyces diastatochromogenes tz3-1 had good inhibitory effects on the mycelial growth of the f...

Embodiment 3

[0029] Example 3: (Field Control Effect Measurement of Amylase Streptomyces Chromogenes tz3-1 Metabolites)

[0030] Select 100%, 50% amylase Streptomyces chromogenes tz3-1 metabolites and 50% carbendazim as the test agents for field control efficacy determination, clean water is the negative control, and each treatment field is treated with clean water The negative control was used as an interval field. On the 7th day, the incidence was investigated, the disease index was counted, and the corrected control effect was calculated. According to the severity of the disease, it is divided into 4 grades: grade 0, healthy seedlings; grade 1, at least 25% of the leaves are dead; grade 2, 26% to 50% of the leaves are dead; grade 3, 51% to 75% of the leaves are dead; level, 76% to 100% of the leaves are dead. Disease index=[∑(number of diseased plants at all levels×representative value corresponding to each level) / (total number of plants under investigation×representative value of the...

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Abstract

The invention discloses the application of an amylase Streptomyces diastatochromogenes metabolite in preventing and treating Dendrobium officinale diseases, and relates to the field of microorganism technology. tz3‑1, deposited on December 3, 2019, deposit accession number CGMCC No.19069. The most notable feature of the present invention is that the liquid fermentation metabolites of the strain can simultaneously inhibit the pathogenic bacteria of Dendrobium officinale (Colletotrichum gloeosporioides), the pathogenic bacteria of root rot (Fusarium chlamydosporum), the pathogenic bacteria of bacterial blight (Alternaria alternata), and the pathogenic bacteria of leaf spot (Cladosporium). cladosporioides) and Sclerotium delphinii hyphae growth. Field experiments showed that the metabolites of this strain were slightly better than conventional chemical pesticides in the control of Dendrobium officinale blight.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of a metabolite of amylase Streptomyces chromogenes in preventing and treating diseases of Dendrobium officinale. Background technique [0002] Dendrobium officinale (Dendrobium officinale) is a perennial herb of the genus Dendrobium orchidaceae, the main medicinal part is fresh or dried stems, and it is a very precious Chinese medicinal material. Traditional medicine believes that Dendrobium officinale has the functions of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat, and improving immunity. Modern pharmacology has found that Dendrobium officinale has a good regulatory effect on the digestive system, immune system, diabetic vascular disease, etc., and has anti-oxidation, anti-aging, anti-fatigue, anti-tumor, and liver protection. It is reported that its main active substances are sesquitier alkaloids, mucus, stero...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/28A01P3/00C12P1/04C12R1/525
CPCA01N63/28C12P1/04
Inventor 申屠旭萍宋阳曹瑱艳俞晓平许益鹏刘光富
Owner CHINA JILIANG UNIV
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