Method for promoting crop growth and disease resistance by synergistic use of multiple microbial agents

A technology for crop growth and multi-bacterial agent, applied in the agricultural field, can solve problems such as the failure to realize the interaction between microorganisms and metabolites and crops, and the failure to realize the whole-process protection of crops, so as to promote the development of crop roots, improve disease resistance, promote effect of growth

Active Publication Date: 2021-04-16
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the biological agents are complexes of various microorganisms, and the method of use is also used in the early stage of the occurrence of diseases and insect pests. However, different growth stages of crops have different requirements for microorganisms. The current conventional one-time application method cannot realize the combination of microorganisms and metabolites with crops. The interactive relationship between crops cannot achieve full protection of crops

Method used

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  • Method for promoting crop growth and disease resistance by synergistic use of multiple microbial agents
  • Method for promoting crop growth and disease resistance by synergistic use of multiple microbial agents
  • Method for promoting crop growth and disease resistance by synergistic use of multiple microbial agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of 2 billion CFU / gram Bacillus subtilis KN-527 granules, 500 million CFU / gram Paenibacillus polymyxa KN-03 granules, 2 billion CFU / gram Bacillus subtilis KN-42 and 500 million CFU / gram polymyxa For Paenibacillus KN-03 composite granules, apply the above-mentioned granules in holes around the root system of citrus trees, take samples 90 days after hole application, and measure the number of Bacillus subtilis and Paenibacillus polymyxa in the soil. The specific results are shown in the table 1.

[0032] Determination of the number of subtilis and Paenibacillus polymyxa in table 1 soil

[0033]

[0034] From the above test results, it can be seen that Bacillus subtilis KN-42 can colonize and survive in soil, and the proliferation and survival ability of Paenibacillus polymyxa KN-03 is relatively poor. When KN-03 was compounded and used at the same time, the proliferation of its subtilis and Paenibacillus polymyxa was lower than the number of spores when it ...

Embodiment 2

[0036] The test set up six treatments, the first treatment was 500 million CFU / gram Paenibacillus polymyxa KN-03 suspension 100 times dilution for 1 hour, and the second treatment was 500 million CFU / gram Paenibacillus polymyxa KN-03 suspension 100 Soak the seeds in the double dilution solution for 1 hour + add 500 million CFU / gram of Bacillus subtilis KN-42 granules to the base fertilizer, and soak the seeds in the 100-fold dilution solution of 500 million CFU / gram of Bacillus subtilis KN-42 suspension for 1 hour in the third treatment, and 100 in the fourth treatment 100 million CFU / gram Bacillus amyloliquefaciens KN-527 suspension 100-fold dilution for 1 hour, treatment five is 10 billion CFU / gram Bacillus amyloliquefaciens KN-527 suspension 100-fold dilution soaking for 1 hour + adding 500 million CFU / to the base fertilizer Bacillus subtilis KN-42 granule, treatment six is ​​blank control. The soaked tomato seeds were dried and sown in nutrient pots, and 30 seedlings were...

Embodiment 3

[0042] Use 500 million CFU / gram Paenibacillus polymyxa KN-03 suspension, 10 billion CFU / gram Bacillus subtilis KN-42 suspension, 10 billion CFU / gram Bacillus amyloliquefaciens KN-527 suspension against rice sheath blight , peanut blight and other pathogenic bacteria were subjected to plate confrontation test, and the test results are shown in Table 3.

[0043] Table 3 Antibacterial effect table of Bacillus amyloliquefaciens etc.

[0044]

[0045] It can be seen from the test that Bacillus amyloliquefaciens KN-527 has inhibitory effect on a variety of pathogenic bacteria, and its inhibitory effect is better than that of Bacillus subtilis KN-42 and Paenibacillus polymyxa KN-03. Surface spray to control various diseases.

[0046] Based on the rapid proliferation of Bacillus subtilis in the soil, Bacillus subtilis becomes the dominant flora in the soil, which provides a good soil micro-ecological environment for the proliferation of Paenibacillus polymyxa, Paenibacillus polymy...

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Abstract

The invention discloses a method for promoting crop growth and disease resistance through synergistic use of multiple microbial agents, and belongs to the field of agricultural planting. According to the method, different microbial agents are used in different growth periods of crops, and firstly, a microbial agent containing bacillus subtilis and base fertilizer are applied at the same time; secondly, during sowing, paenibacillus polymyxa is used for seed dressing, or during transplanting, a paenibacillus polymyxa suspending agent is used for root dipping, and after seedlings emerge neatly or are transplanted and recovered, a paenibacillus polymyxa microbial agent is used for performing flushing application again; and finally, bacillus amyloliquefaciens is sprayed to the overground parts of the crops. With adoption of the method, different action mechanisms of microorganisms and metabolites are fully utilized, synergistic interaction is achieved, crop growth can be remarkably promoted, the crops are induced to generate stress resistance, diseases are controlled, and green prevention and control of the crops are achieved.

Description

technical field [0001] The invention belongs to the field of agriculture, and in particular relates to a method for promoting crop growth and disease resistance through the synergistic use of multi-bacteria agents. Background technique [0002] The use of chemical pesticides and inorganic fertilizers has effectively promoted the increase in crop production and played a very important role in the rapid development of my country's agriculture. However, due to the long-term large-scale application of inorganic fertilizers and chemical pesticides, on the one hand, the quality of agricultural products in my country has declined; , has brought a series of problems such as food safety and environmental pollution. [0003] In response to the above problems, the country is vigorously advocating the use of biological pesticides to control pests and diseases, and the application of organic fertilizers to improve soil. After the promotion of biological control and organic fertilizers in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01B79/02A01C1/06A01G13/00A01N63/22C09K17/14A01P1/00A01P3/00A01P21/00C09K101/00
Inventor 姜勇王晓辉刘华梅孙刚忠李青张晓明常登科周波霍瑞胡虓张芊王加冕李飞
Owner WUHAN KERNEL BIO-TECH CO LTD
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