In-vitro experiment device capable of applying parameterized electric field and temperature control method of in-vitro experiment device

Abstract

The invention provides an in-vitro experiment device capable of applying a parameterized electric field. The in-vitro experiment device comprises culture dishes (8) for accommodating in-vitro cultured biological tissues, an even number of electrode plates (14) which are arranged on the inner walls of the culture dishes (8) and used for applying an electric field to a culture solution, and a structural bracket (15) which is used for fixing the culture dishes (8) and the electrode plates (14). Compared with the prior art, the in-vitro experiment device has the advantages that the temperature range of a culture medium suitable for cell growth is set, and the heat production amount is intelligently adjusted according to the temperature change of the culture medium, so that the temperature during heat production and heat dissipation balance is within the set range, and cell culture is facilitated. Electric fields with different parameters can be applied according to the set different environment temperatures. The culture dishes are made of a material with high heat conductivity, and heat dissipation can be accelerated in the cooling process.

Description

technical field [0001] The invention belongs to the field of in vitro experimental equipment, and in particular relates to an in vitro experimental research device and a temperature control method for applying a parameterized electric field to single-cell organisms, isolated tissues or cells. Background technique [0002] Tumor Therapeutic Electric Fields (TTFields) is a mature treatment that inhibits cell proliferation by interfering with cell mitosis. This low-intensity, medium-frequency, alternating electric field is used to intervene in a variety of tumor diseases and is a clinically effective cancer treatment. Many basic studies at home and abroad have confirmed the biological effects of TTFields. The results of cell experiments show that TTFields can regulate the expression of biochemical molecules in tumor cells, interfere with the division cycle of tumor cells, inhibit the proliferation of tumor cells and promote the apoptosis of tumor cells. The results of animal ...

AI Technical Summary

Problems solved by technology

In addition, the in vitro culture of biological tissues has relatively strict requirements on temperature conditions (usually maintained between 36-37°C), and the action of an electric field on the medium will generate a large amount of heat, resulting in an increase in temperature

Many previous in vitro research results did not take into account the influence of heat production, and biological tissues were cultured in an abnormal temperature environment, so the experimental results lacked reference value and comparability

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