Application of C/EBP alpha and CD68 as prognostic markers in preparation of liver cancer prognostic prediction kit

Abstract

The invention discloses an application of C / EBP alpha and CD68 as prognostic markers in preparation of a liver cancer prognostic prediction kit. Researches show that the in-situ differentiation and maturation state of mononuclear / macrophages in liver cancer tissues can be judged according to the in-situ co-localization condition of C / EBP alpha and CD68 in the tissues, so that the in-situ immune response condition of the tissues of liver cancer patients is determined, clinicians are helped to better judge the prognosis survival condition of the patients, and an immunological theoretical basis is provided for selecting proper individualized treatment. The invention also provides an application of the double-antibody combined fluorescence detection kit for detecting the differentiation maturity of mononuclear / macrophages in liver cancer tissues, the double-antibody combined fluorescence detection kit comprises a first antibody and a second antibody, the first antibody is a combination of C / EBP alpha and CD68 antibodies, and the second antibody is a fluorescently labeled second antibody.

Description

technical field [0001] The invention relates to the technical field of disease prognosis diagnosis, in particular to the application of C / EBPa and CD68 as prognosis markers in the preparation of liver cancer prognosis prediction kits. Background technique [0002] Liver cancer is one of the most common malignant tumors with the worst prognosis in my country, most of which develop from long-term chronic inflammation. Regardless of the origin of liver cancer, it can lead to an imbalance in the immune response. A large number of immune cells, together with the cytokines and metabolites produced by them, cause gene mutations and changes in the epigenetic level of liver parenchymal cells, and gradually evolve into malignant tumor cells. Monocytes / macrophages are one of the most abundant leukocytes, which play a very important role in regulating the occurrence, progression and prognosis of liver cancer. Monocytes / macrophages are both effector cells and antigen-presenting cells. ...

AI Technical Summary

Problems solved by technology

However, for these monocytes, the fate of differentiation, degree of differentiation, relationship with functional phenotype, and the specific mechanism of how tumors participate in these processes are not clear

So far, there are no related techniques or indicators that can reflect the differentiation, maturation and proliferation of monocytes / macrophages in situ in liver cancer tissues

At present, there are no related reports on the use of C / EBPa and CD68 to reflect the differentiation and maturation status of monocytes / macrophages in situ in liver cancer tissues, so as to predict the prognosis and survival of patients with liver cancer

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