Separation equipment for cell exosome and using method of separation equipment

Abstract

The invention discloses separation equipment for cell exosome and a using method of the separation equipment, and belongs to the technical field of cell separation. The separation equipment comprises a shell, wherein an end cover is hinged to the top of the shell, a rotating disc is arranged at the middle of the interior of the shell, and inserting grooves used for containing a test tube containing cells are formed in the rotating disc; and a lifting mechanism for controlling the rotating disc to lift is arranged below the rotating disc. By arranging the lifting mechanism, a driving mechanism, a linkage mechanism, a sealing mechanism and a limiting mechanism, when an experimenter opens the end cover, the linkage mechanism and the lifting mechanism are used for lifting the rotating disc, the experimenter can conveniently take and place the test tube, when the end cover is closed, the rotating disc descends to a working position, and then the upper end of the rotating disc is sealed through the sealing mechanism; and finally, the end cover is limited through the limiting mechanism, the experimenter does not need to take out the rotating disc and seal the test tube after the test tube is placed, the operation of the experimenter is facilitated, and the preparation time before an experiment is saved.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to a device for separating exosomes from cells and a method for using the same. Background technique [0002] Exosomes are nanovesicles secreted by cells with a lipid bilayer structure, with a particle size distribution of 30-150nm, containing lipids, proteins, nucleic acids and other substances, which are very important in the communication between cells role. Existing studies have confirmed that exosomes are widely involved in various physiological and pathological processes, and can be used as biomarkers for disease screening and diagnosis. Therefore, the isolation and enrichment of exosomes is of great significance in both clinical and scientific research fields. [0003] When the existing centrifuge is in operation, the experimenter needs to open the end cover, then take out the turntable for storing the test tubes, then the experimenter puts the prepared test tubes f...

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of separation equipment of cell exosomes and its use method, in order to solve the problem that the existing centrifuge in the above-mentioned background technology needs to be put on and off when taking the installation turntable to disassemble the test tube during operation. The transfer and installation of the turntable of the test tube is more troublesome, which brings great inconvenience to the staff. During the process of transfer and installation, it is easy to cause the test tube to slip and cause work errors.

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