Fixed bed type bioreactor

Abstract

The invention relates to the technical field of bioreactors for culturing adherent cells and suspension cells with low shear force, in particular to a fixed bed type bioreactor. The fixed bed type bioreactor comprises a tank body and a carrier column, wherein the upper part of the tank body is hermetically connected with a tank cover; a magnetic stirring base is arranged at the lower part in the tank body; a drainage hole is formed in the bottom of the magnetic stirring base; a magnetic stirrer for rotationally stirring water is installed in the magnetic stirring base; the carrier column is detachably installed above the magnetic stirring base; and the carrier column is filled with a plurality of fibrous carrier sheets. The magnetic stirrer drives a culture medium to flow in from the drainage hole in the bottom, pass through the carrier sheets in the carrier column, finally flow out from the top of the carrier column and return to the tank body through rotary stirring to form a circulating liquid flow direction in the culture medium. Aiming at the defects in the prior art, the problems that part of cells difficultly grow in the bioreactor and part of suspension cells produce toxin in a common STR reactor or the protein expression quantity is low are solved.

Description

technical field [0001] The patent of the invention relates to the technical field of bioreactors for cultivating adherent cells and suspension cells with low shear force, in particular to a fixed bed bioreactor. Background technique [0002] Stirred tank bioreactor (STR) is often used in traditional large-scale cell culture, that is, cells are attached to microcarriers, fiber carrier sheets or fully suspended by themselves, and are suspended and grown in the bioreactor under the stirring of the bioreactor stirring paddle. . However, the shear force brought by this form of agitation can lead to cell damage or death. Some of the adherent cells that have been used for production are difficult to grow in traditional STR reactors, and can only be cultured in traditional static culture, which is difficult to meet the needs of large-scale culture. There are also some whole suspension cells that can grow, but the protein expression level is low under the action of shear force. At...

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Problems solved by technology

[0003] Aiming at the deficiencies of the prior art, the present invention provides a fixed-bed bioreactor with reliable performance, which solves the problem that some cells are difficult to grow in the bioreactor and some suspended cells produce toxin or have low protein expression in the common STR reactor The problem

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