Method for establishing fingerprint spectrum of Draba nemorosa and Chinese date lung-purging granules

A technology of fingerprints and standard fingerprints, applied in the field of establishment of HPLC fingerprints of Tingli Dazao Xiefei Granules, to achieve the effects of many characteristic peaks, good baseline separation, and good peak shape

Active Publication Date: 2021-04-30
LUNAN PHARMA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for accurate evaluation of both qualitative features such as particle size distribution (PM) or specific chemical properties like nitrogen content by measuring certain physical parameters that are associated with these attributes during processing steps on frozen samples collected from different parts of China's citrus plants called Citrils Louriscus Benthoselans Cantor). These techniques help identify defective products quickly without relying solely upon their appearance alone.

Problems solved by technology

This patented describes an improved way to check if certain drugs have been taken properly during treatment. One technical problem addressed is how to accurately determine whether these drugs were safe when given them through various ways such as extractive techniques like tincture injection (TZ), crystal powder production processes, dried liquor evaporation process, concentrated liquid culture technique, and other means that may affect their efficacy over time.

Method used

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  • Method for establishing fingerprint spectrum of Draba nemorosa and Chinese date lung-purging granules
  • Method for establishing fingerprint spectrum of Draba nemorosa and Chinese date lung-purging granules
  • Method for establishing fingerprint spectrum of Draba nemorosa and Chinese date lung-purging granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Establishment of HPLC Standard Fingerprint of Tingli Dazao Xiefei Granules

[0028] 1 Instruments and reagents

[0029] 1.1 Instrument

[0030] Agilent 1260 high performance liquid chromatography (USA): DAD detector, quaternary low-pressure gradient pump, AgilentOpen Lab chromatography workstation.

[0031] 1.2 Reagent

[0032] Tingli Dazao Xiefei Granules were provided by Lunan Houpu Pharmaceutical Co., Ltd., and the sample batch numbers are shown in Table 1. Acetonitrile was chromatographically pure, water was double distilled water, and other reagents were analytically pure.

[0033] Table 1 Batch No. of Test Samples of Tingli Dazao Xiefei Granules

[0034]

[0035]

[0036] 2 Methods and Results

[0037] 2.1 Chromatographic conditions: chromatographic column: Agilent ZORBAX SB C18 (4.6×250mm, 5μm) column; mobile phase: acetonitrile as mobile phase A, 0.12% phosphoric acid aqueous solution as mobile phase B, gradient as shown in the table below ...

Embodiment 2

[0056] Example 2 Establishment of HPLC Standard Fingerprint of Tingli Dazao Xiefei Granules

[0057] 1 Instruments and reagents

[0058] 1.1 Instrument

[0059] Agilent 1100 high performance liquid chromatography (USA): DAD detector, quaternary low-pressure gradient pump, AgilentOpen Lab chromatography workstation.

[0060] 1.2 Reagent

[0061] Tingli Dazao Xiefei Granules were provided by Lunan Houpu Pharmaceutical Co., Ltd., and the sample batch numbers are shown in Table 4; acetonitrile was chromatographically pure, water was double distilled water, and the rest of the reagents were analytically pure.

[0062] Table 4 Batch numbers of test samples of Tingli Dazao Xiefei Granules

[0063]

[0064] 2 Methods and results

[0065] 2.1 Chromatographic conditions: Chromatographic column: Agilent ZORBAX SB C18 (4.6×250mm, 5μm) column; mobile phase: acetonitrile as mobile phase A, 0.18% by volume phosphoric acid aqueous solution as mobile phase B, gradient as shown in the ta...

Embodiment 3

[0071] Example 3 Establishment of HPLC Standard Fingerprint of Tingli Dazao Xiefei Granules

[0072] 1 Instruments and reagents

[0073] 1.1 Instrument

[0074] Agilent 1100 high performance liquid chromatography (USA): DAD detector, quaternary low-pressure gradient pump, AgilentOpen Lab chromatography workstation.

[0075] 1.2 Reagent

[0076] Tingli Dazao Xiefei Granules were provided by Lunan Houpu Pharmaceutical Co., Ltd., and the sample batch numbers are shown in Table 5; acetonitrile was chromatographically pure, water was double distilled water, and other reagents were analytically pure.

[0077] Table 5 Batch numbers of test samples of Tingli Dazao Xiefei Granules

[0078]

[0079] 2 Methods and Results

[0080] 2.1 Chromatographic conditions: chromatographic column: Agilent ZORBAX SB C18 (4.6×250mm, 5μm) column; mobile phase: acetonitrile as mobile phase A, 0.12% phosphoric acid aqueous solution as mobile phase B, gradient as shown in the table below Elution: ...

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Abstract

The invention discloses a method for establishing an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of Draba nemorosa and Chinese date lung-purging granules. The method comprises the following steps: preparing a test solution, determining HPLC chromatographic conditions and manufacturing an HPLC standard fingerprint spectrum. The invention also discloses an HPLC standard fingerprint spectrum of the Draba nemorosa and Chinese date lung-heat-purging granules obtained by the method. The fingerprint spectrum has 27 common peaks. The quality detection method disclosed by the invention is simple and convenient to operate, high in stability and good in reproducibility, the number of characteristic peaks of the obtained chromatogram is large, the quality of the Draba nemorosa and Chinese date lung-purging granules can be comprehensively evaluated and controlled by comparing common peaks of standard fingerprint chromatograms, the internal quality of a preparation can be accurately evaluated, and the safety and effectiveness of clinical medication are ensured.

Description

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Claims

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Application Information

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Owner LUNAN PHARMA GROUP CORPORATION
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