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Tissue culture method for nymphaea hybrid

A technique of tissue culture and perfume, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of flower quality difference, nine-grade perfume lotus flower quality difference, low economic benefits, etc., and achieve stable quality and good flower quality Effect

Pending Publication Date: 2021-05-11
海南科技职业大学
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AI Technical Summary

Problems solved by technology

It is difficult to guarantee the quality and quantity of Jiupin Perfume Lotus, which has become a bottleneck in the development of Jiupin Perfume Lotus
In order to meet the ever-increasing demand for Nine-Pin Perfume Lotus, it is usually adopted to introduce Nine-Pin Perfume Lotus from other places to expand its planting area. However, the introduction of Nine-Pin Perfume Lotus in the subtropical zone is planted in ordinary simple greenhouses, which lacks aesthetic appreciation and is not conducive to cr

Method used

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  • Tissue culture method for nymphaea hybrid
  • Tissue culture method for nymphaea hybrid
  • Tissue culture method for nymphaea hybrid

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Embodiment Construction

[0015] In Haikou City, Hainan Province, the scientific research team cultivated Jiupin Perfume Lotus through artificial tissue culture. The operation is as follows: the first step is to take the rhizome of Jiupin Perfume Lotus, cut off the terminal bud, lateral bud and part of the rhizome tissue, and clean it Finally, infiltrate with 75% alcohol for 60 seconds, rinse with sterile water, put it into 1% NaClO solution for disinfection for 20 minutes, rinse with sterile water for 6 times, remove residual NaClO, and dry the explant surface with sterile filter paper. moisture;

[0016] In the second step, the explants are inoculated on the germination medium, and the composition of the medium is MS+6-BA 1 mg / L+IBA 0.5 mg / L+4% sucrose+0.8% agar. The culture temperature is 25°C, the light is 12h / d, and the light intensity is 2000lx;

[0017] The third step is to germinate and culture for about 12 days, divide the clustered buds into single buds and transfer them to the proliferation...

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Abstract

The invention discloses a tissue culture method for a nymphaea hybrid. The tissue culture method comprises the following steps of conducting pretreating, inoculating an explant on a germination culture medium for tissue culture, dividing multiple shoots into single buds, transferring the single buds to a proliferation culture medium for culture, inoculating into a rooting culture medium for inducing growth of roots, exercising seedlings, domesticating and the like. The nymphaea hybrid obtained by the method is stable in quality and good in flower appearance, and flowers have aesthetic feeling and ornamental value.

Description

technical field [0001] The present invention relates to a kind of plant vegetative propagation method, relate to a kind of method of Nine Pins Perfume lotus tissue culture more specifically. Background technique [0002] Jiupin Perfume Lotus is a large water lily. Elegant and noble fragrance, beautiful color, long flowering period. On the basis of the introduction of American lotus in 1979, Taiwan's garden department has gradually developed nine color systems including gold, yellow, purple, blue, red, green, red, and white after years of cultivation. Therefore, it is also called "Nine Grade Water Lotus". Jiupin perfume lotus does not bear lotus pods and does not grow lotus roots. It can only rely on asexual reproduction, and its growth and reproduction are slow. There are few plants in South China. It was only introduced in Hainan Province in recent years. It is currently planted in Haikou, Chengmai, and Qionghai. The planting area increasing year by year. Nine-Pin Perfu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 罗海希
Owner 海南科技职业大学
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