Self-adapting-brightness-controlled online biomass detection device

Abstract

The invention relates to a self-adapting-brightness-controlled online biomass detection device. The device comprises a light source, a light signal receiver, a mounting rack and a control circuit board, wherein the light source and the light signal receiver are mounted on the mounting rack; the control circuit board is electrically connected with the light source and the light signal receiver; the light source is used for emitting light signals; the light signal receiver is used for receiving light signals diffused or transmitted by a culture solution to be detected; and the control circuit board is used for carrying out signal processing according to the light signals emitted from the light source and the received light signals so as to obtain light density and adjusting brightness of the light source in a self-adapting manner.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a non-contact biomass online detection device. Background technique [0002] In bioengineering or fermentation engineering, a certain amount of microorganisms are often inoculated in the medium, and then kept in a specific environment for cultivation. Usually, the growth status of microorganisms needs to be monitored during the cultivation process. The most common method in laboratories today is the optical density (OD) measurement method. The optical density measurement method is to inject the light signal of the light source into the culture medium for cultivating microorganisms, and then receive the scattered or transmitted light signal, and then process the signal to indirectly obtain the growth state of the microorganism. [0003] The inventor found in the research that the optical density measurement method in the prior art is mainly an off-line measurem...

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Problems solved by technology

[0003] The inventor found in the research that the optical density measurement method in the prior art is mainly an off-line measurement, that is, during the cultivation of microorganisms, when the experiment needs to measure the growth state of microorganisms, a part of the culture is taken out from the petri dish. In this way, the operation of the experimenter is more frequent, and it is easy to greatly increase the risk of introducing impurities or bacteria into the culture solution during multiple operations.

On the other hand, in the prior art, optical density measurement devices mainly include scattering (reflective) or transmissive ones, no matter what kind of device, the light source of which emits light with a specific brightness is only suitable for a small optimal range of optical density In the process of microbial cultivation, as the biomass changes, the light signal obtained by scattering or transmission will always change accordingly, and the range of optical density will also change again, that is, a specific device with fixed light brightness Not very suitable for long-term biological culture, and its optimal measurement optical density range is limited

Even if some measuring devices provide the brightness adjustment function, it is manually adjusted, and the brightness is adjusted manually according to the growth status of microorganisms. This has high subjective factors, requires rich experimental experience, and is frequently operated.

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