Molecular markers for auxiliary diagnosis of viral meningitis as well as application and kit of molecular markers

Abstract

The invention discloses molecular markers for auxiliary clinical diagnosis of viral meningitis as well as application and a kit of the molecular markers. Firstly, systematic gene chip analysis is carried out on two groups of cerebrospinal fluid samples of viral meningitis and normal control by a miSeq high-throughput sequencing technology, differential miRNA expression is screened, the screened differential expression miRNA is further verified one by one by an RT-qPCR technology, and finally, it is found that miR-RNA 18_41385, hsa-miR-486-5p, hsa-miR-4497 and the hsa-miR-3178 can be used as specific markers for early auxiliary clinical diagnosis of the viral meningitis. The markers can also be used for differential diagnosis of other meningitis. The kit has the advantages of being good in stability, simple and convenient in detection method, high in sensitivity, specific and the like, achieves effective supplementation of an existing clinical viral meningitis diagnosis method and rapid definite diagnosis of viral meningitis patients, and is of great significance in reducing the death rate of the viral meningitis and improving the treatment effect and prognosis.

Description

technical field [0001] The invention belongs to the technical field of viral meningitis diagnosis, and in particular relates to a cerebrospinal fluid-specific molecular marker for viral meningitis diagnosis, its application and a matching detection kit. Background technique [0002] In my country and even the world, viral meningitis is a relatively common and serious infectious disease of the central nervous system, which can be caused by various viruses invading the meninges. Viral meningitis can cause fever, headache, vomiting and, in severe cases, permanent brain damage and even death. The clinical manifestations are very similar to other types of central nervous system infections, and need to be identified in combination with medical history, cerebrospinal fluid examination, and etiological analysis; The symptoms shown are also similar to those of viral meningitis, so many patients with viral meningitis are often misdiagnosed and mistreated in the early stage, missing t...

AI Technical Summary

Problems solved by technology

However, the process of meningitis is often limited to the central and deep layers of the temporal lobe. If the materials are not obtained properly, the purpose will not be achieved. Moreover, there are many types of viruses, and the number of new or unexplained viral meningitis is increasing. It is difficult to isolate viruses routinely. And there is a lack of specific and sensitive molecular biological detection methods

①Using brain tissue for virus isolation is currently recognized as the most reliable method for diagnosis, but it cannot be used as a routine diagnosis method. The first is the time of diagnosis. Some viruses (such as herpes simplex virus) can disappear from the nerve tissue in a short time. Secondly, biopsy May also cause damage to the brain, occasionally causing massive bleeding or concurrent infection

③ Detection of virus genes in cerebrospinal fluid by PCR technology is an emerging molecular gene detection technology. The detection method is fast and convenient. However, the content of virus or its antigen in cerebrospinal fluid CSF is very small, resulting in a very low positive rate of detection, prone to false positives, and relies on genome sequencing platforms

④ Immunological and serological methods also have certain limitations, and limited by laboratory conditions, they have not been widely carried out, and the diagnosis is often non-specific

[0006] (3) Imaging techniques such as CT and MRI have a very low positive rate in the early diagnosis of viral meningitis. Generally, it is difficult to find lesions or have no abnormal manifestations in the early stage. The EEG wave pattern of viral meningitis is mainly diffuse Irregular high-amplitude slow waves. For focal lesions, the EEG usually shows that one side is heavier, which can help to judge the lesion site. In patients with viral meningitis, brain function abnormalities are earlier than structural abnormalities, and the definition of the lesion edge is not clear. First, it is easy to cause confusion with other diseases, and in the early stage of the onset, its imaging specificity is not obvious. The accuracy of CT, MRI and other imaging techniques for the diagnosis of viral meningitis is mainly in the middle and early stages of the onset. Late stage, and its examination is expensive, not suitable as a routine screening method for the general population

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