A kind of method and application for distinguishing northern Bupleurum and Tibetan Bupleurum
A technology of Tibetan Bupleurum and Bupleurum, which is applied in the field of identification of Chinese herbal medicines, can solve the problems that the identification of traits is greatly affected by subjective factors and the analysis period is long.
Active Publication Date: 2022-07-22
广东省药品检验所
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Problems solved by technology
In related technologies, the methods for identifying Bupleurum radix are mostly character identification, such as thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), high-performance liquid chromatography-mass spectrometry (HPLC-MS), etc., but character identification is subject to subjective Factors have great influence, while methods such as TCL, HPLC and HPLC-MS require complex pretreatment of medicinal materials, and the analysis cycle is longer
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[0039] In order to make the invention purpose, technical solutions and technical effects of the present invention clearer, the present invention will be further described in detail below with reference to the specific embodiments. It should be understood that the specific embodiments described in this specification are only for explaining the present invention, rather than for limiting the present invention.
[0040] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial sources.
[0041] Experimental Materials
[0042] (1) Experimental equipment:
[0043] Headspace-Gas Chromatography-Ion Mobility Spectrometer (German G.A.S. Company) and supporting analysis software LAV (laboratory analytical viewer) and 3 plug-ins Reporter, GalleryPlot, Int DynamicPrincipal Components Analysis (Int Dynamic PCA).
[0044] (2) Experimental samples:
[0045] Northern Bupleurum medicinal material ...
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Abstract
The invention discloses a method for identifying Bupleurum chinensis and Bupleurum chinensis and application thereof. The steps include: taking a sample of Bupleurum chinensis and incubating for 30-40 minutes, and using headspace-gas chromatography-ion mobility spectrometry for detection. Among them, 4 volatile characteristic peaks of Beibu Bupleurum and 4 volatile characteristic peaks of Tibetan Bupleurum were obtained by MXT-WAX column detection, and the total proportion of PC-1 and PC-2 in principal component analysis was 91%. Compared with the prior art, the identification method in the present invention does not require sample pretreatment, has short analysis period, low detection cost, and has a portable instrument.
Description
technical field [0001] The invention relates to an identification method for traditional Chinese medicinal materials, in particular to a method for identifying Beibu Bupleurum and Tibetan Bupleurum and its application. Background technique [0002] Bupleurum chinense is the dried root of Bupleurum chinense, and it is one of the most commonly used anti-exogenous medicines in clinical practice. The effect of reducing muscle fever and raising yang qi is used for symptoms such as cold and fever, cold and heat exchanges, malaria, liver stagnation and qi stagnation. Tibetan Bupleurum (B.marginatum var. Stenophyllum.) is mainly produced in Gansu, and its antipyretic and anti-inflammatory effects are similar to those of Beibu Bupleurum, but the liver protection effect is not obvious, and it has pharmacological toxicity. [0003] Bupleurum has a wide variety of chemical components, and different authentic medicinal materials have different content of active components, which is dire...
Claims
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IPC IPC(8): G01N30/02G01N30/72G01N30/86
CPCG01N30/02G01N30/72G01N30/8631G01N30/8686
Inventor 刘亚雄罗卓雅乔莉陈馥
Owner 广东省药品检验所