Method for widening tetraploid wheat genetic variation

A technique of genetic variation and tetraploidy, applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problems of low frequency of beneficial mutations, high cost of chemical and physical mutations, environmental pollution, etc., to achieve The effects of high mutagenesis efficiency, rich mutagenesis types, and high mutation frequency

Pending Publication Date: 2021-05-18
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for widening the genetic variation of tetraploid wheat to solve the problem of high chemical and physical mutagenesis costs for creating genetic variation in tetraploid wheat in the prior art, and the frequency of beneficial mutations is low. There are also technical issues with the potential risk of environmental pollution

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  • Method for widening tetraploid wheat genetic variation
  • Method for widening tetraploid wheat genetic variation
  • Method for widening tetraploid wheat genetic variation

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for widening the genetic variation of tetraploid wheat, comprising the steps of:

[0037] (1) The genetically stable parental tetraploid wheat AABB was sexually crossed with the genetically stable diploid DD and bagged, and triploid immature embryos ABD were obtained 12-15 days after pollination;

[0038] (2) The triploid immature embryos ABD obtained in step (1) are cultured in vitro in the laboratory using tissue culture technology to obtain ABD plant seedlings;

[0039] Wheat immature embryos are cultured in vitro using MS solid medium. The specific formula of MS solid medium is as follows:

[0040] 1) 500ml of 100× iron salt mother liquor (1.39g of FeSO 4 ·7H 2 O, 1.86 g Na 2 EDTA·2H 2 O);

[0041] 2) 500ml of 20×macroelement mother liquor (16.5g of NH 4 NO 3 , 19 g KNO 3 , 1.7 g KH 2 PO 4 , 3.7 g MgSO 4 ·7H2 O, 3.32 g anhydrous CaCl 2 );

[0042] 3) 100×trace element mother solution 200ml (16.6 mg KI, 124 mg H 3 BO 3 , 338 mg MnSO 4 ·H 2 O...

Embodiment 2

[0062] The difference with embodiment 1 is:

[0063] In step (3), the method for performing chromosome doubling treatment on ABD seedlings is: soaking ABD seedlings with a mixed solution of colchicine and dimethyl sulfoxide (the preparation method of the mixed solution of colchicine and dimethyl sulfoxide is: First prepare a colchicine solution with a mass percentage concentration of 0.06%, and then prepare a volume percentage concentration of 1.2% dimethyl sulfoxide solution; finally, mix the two solutions evenly, which is a mixture of colchicine and dimethyl sulfoxide solution); at the same time, use an oxygen pump to maintain an oxygenated state at the bottom of the mixed solution (it is enough to keep the mixed solution with continuous bubbles during the entire experiment); under the environmental conditions of a temperature of 20 ° C and light (fluorescent lamp irradiation), treat for 18 hours , after flushing for 2.2 hours, the chromosome doubling treatment of ABD seedli...

Embodiment 3

[0066] The difference with embodiment 1 is:

[0067] In step (3), the method for performing chromosome doubling treatment on ABD seedlings is: soaking ABD seedlings with a mixed solution of colchicine and dimethyl sulfoxide (the preparation method of the mixed solution of colchicine and dimethyl sulfoxide is: First prepare a colchicine solution with a mass percentage concentration of 0.04%, and then prepare a volume percentage concentration of 1.8% dimethyl sulfoxide solution; finally, mix the two solutions evenly, which is a mixture of colchicine and dimethyl sulfoxide solution); at the same time, use an oxygen pump to maintain an oxygenated state at the bottom of the mixed solution (it is enough to keep the mixed solution with continuous bubbles during the entire experiment); under the environmental conditions of a temperature of 20 ° C and light (fluorescent lamp irradiation), treat for 22 hours , after flushing for 1.8 hours, the chromosome doubling treatment of ABD seedli...

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Abstract

The invention discloses a method for widening tetraploid wheat genetic variation. The method comprises the following steps that parent tetraploid wheat AABB is utilized to hybridize with diploid aegilops tauschii DD, in-vitro culture is carried out on a hybrid young embryo ABD to obtain an ABD plant seedling, then chromosome doubling treatment is carried out to obtain low-generation artificially synthesized hexaploid wheat AABBDD, the low-generation artificially synthesized hexaploid wheat AABBDD is backcrossed with the same parent tetraploid wheat AABB to obtain a pentaploid wheat material AABBD, the pentaploid wheat material is used for selfing, chromosome behaviors such as chromosome exchange, translocation, inversion, deletion and insertion are generated through chromosome recombination in the selfing process, rich variation types are generated in genetic offspring, and a new tetraploid wheat material composed of different chromosomes is formed. According to the method, the chromosome of the tetraploid wheat can be induced to generate new structural variation in a relatively short time, and the method is high in beneficial mutation efficiency, rich in mutation type, environment-friendly, safe and low in cost.

Description

technical field [0001] The invention belongs to the technical field of new plant strain breeding, and in particular relates to a method for broadening the genetic variation of tetraploid wheat. Background technique [0002] tetraploid wheat ( Triticum turgidum L., 2n=4x=28, AABB) belongs to the annual plant of the Triticea family, is allopolyploid, and contains two subgenomes, the A genome and the B genome. Tetraploid wheat is common wheat ( Triticum aestivum L. 2n=6x=42, AABBDD) has a variety of excellent traits, and can be used as a breeding parent to improve common wheat varieties, thereby improving the yield and quality of wheat varieties, and achieving new breakthroughs in wheat breeding. Durum wheat in tetraploid wheat is the main raw material for producing pasta or macaroni in the world. It has high protein content and hard texture. Its planting area in the world is second only to common wheat. Therefore, creating new mutations of tetraploid wheat and increasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H1/02A01H6/46
CPCA01H1/08A01H1/02A01H6/46
Inventor 杨武云李俊杨凡万洪深刘泽厚王琴杨宁
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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