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Multi-module cooperative control PCR instrument rapid temperature changing device and use method

A technology of collaborative control and temperature change device, applied in chemical instruments and methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the temperature sensor temperature "overshoot, affect the final accuracy, poor temperature control and other problems , to achieve the effect of improving the accuracy

Active Publication Date: 2021-05-25
艾普拜生物科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the traditional PCR instrument temperature control devices on the market are composed of a heating plate, a temperature sensor and a fan radiator. The plate performs repeated heating and cooling process control. This traditional mode of heating and cooling not only takes a long time, but also tends to cause temperature "overshoot" due to the difference in response time of the temperature sensor, resulting in poor temperature control. Good but affects the final accuracy of the PCR reaction

Method used

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  • Multi-module cooperative control PCR instrument rapid temperature changing device and use method
  • Multi-module cooperative control PCR instrument rapid temperature changing device and use method
  • Multi-module cooperative control PCR instrument rapid temperature changing device and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 A rapid temperature change device for PCR instrument with multi-module cooperative control

[0046] like figure 1 and figure 2 As shown, a multi-module cooperatively controlled PCR instrument rapid temperature change device includes heating module I, heating module II, heating module III, a lifting module and a rotating module.

[0047] like image 3 , Figure 4 As shown, heating module I is composed of 96-hole plate mold G (16), temperature sensor R (34), heating film J (35), heat insulation plate L (17), and heat preservation plate O (18); one 96-hole A temperature sensor R (34) runs through the middle of the plate mold G (16), and a heating film J (35) is attached to the bottom; a heating film J (35) is attached to a heat shield L ( 17); the lower part of a heat insulation plate L (17) is close to a heat insulation plate O (18); a 96-hole plate mold G (16), a heating film J (35), and a heat insulation plate L (17), 1 insulation board O (18) are connec...

Embodiment 2

[0053] Example 2 Working steps of a multi-module cooperatively controlled PCR instrument rapid temperature change device

[0054] S1. Working steps of the heating module:

[0055] S1.1 Heating film J (35) or heating film K (40) or heating film W (36) in heating module I, heating module II and heating module III are connected with temperature sensor R (34) or temperature sensor S (45) respectively. ) or the temperature sensor T (46) are connected with the external control circuit at the same time, when the heating film J (35) or heating film K (40) or heating film W (36) respectively feed the 96-hole plate mold G (16) or 96-hole When the plate mold H (38) or 96-hole plate mold U (39) is heated, the temperature sensor R (34) or temperature sensor S (45) or temperature sensor T (46) is constantly feeding back the working status of the heating module in real time;

[0056] S1.2 When the set temperature is reached, the heating film J (35) or the heating film K (40) or the heating ...

Embodiment 3

[0065] Example 3 Detection of temperature overshoot of single-module variable temperature device in two-step PCR reaction

[0066] This embodiment detects the temperature overshoot phenomenon in the two-step PCR reaction of the traditional PCR instrument that adopts a single-module temperature-variable device on the market at present, and the results are shown in Table 1, Table 2, Figure 7 and Figure 8 shown.

[0067] Table 1. Determination of temperature overshoot at 45°C in the two-step PCR reaction of a traditional PCR instrument using a single-module variable temperature device

[0068] Time: year, month, day, hour, minute, second Temperature: °C 2020-08-12 09:49:34 T1:54.9217 2020-08-12 09:49:35 T1:53.2222 2020-08-12 09:49:36 T1:51.3182 2020-08-12 09:49:37 T1:49.757 2020-08-12 09:49:38 T1:48.0088 2020-08-12 09:49:39 T1:46.5613 2020-08-12 09:49:40 T1:44.9518 2020-08-12 09:49:41 T1:43.6233 2020-08-12 09:49:42 T1:42...

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Abstract

The invention provides a multi-module cooperative control PCR instrument rapid temperature changing device. The device is composed of a heating module I, a heating module II, a heating module III, a lifting module and a rotating module. Different temperatures of the three heating modules are set, wherein rapid switching among the three heating modules is completed through cooperative operation of the lifting module and the rotating module at the same time, and the mode of switching different temperature modules is used for replacing the process of heating and cooling of a traditional single heating module. Therefore, the cycle time of the PCR reaction required by the repeated heating and cooling of a single heating module can be shortened by 71-85%, and the phenomenon of sharply varying temperature caused by the heating and cooling process of a single heating module in the traditional PCR instrument is overcome, so that the accuracy of the PCR reaction result is ensured.

Description

technical field [0001] The invention belongs to the field of biochemical equipment, and in particular relates to a multi-module cooperatively controlled PCR instrument rapid temperature change device and a use method. Background technique [0002] PCR (Polymerase Chain Reaction) is a kind of polymerase chain amplification reaction, and various PCR reactions are carried out in the PCR machine at present. The PCR instrument includes the most critical two systems: a photoelectric system and a temperature control system. The photoelectric system is used to collect and convert photoelectric signals, because the PCR reaction generally requires three temperatures of melting 95°C, annealing 55-60°C, and extending 72°C, and the temperature must be continuously raised and lowered during the PCR experiment. 40 or so. Therefore, the temperature conditions required to maintain the PCR reaction and the accuracy of temperature control will directly affect the performance of the PCR instr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/00C12Q1/686
CPCB01L7/52B01L2300/1805C12Q1/686C12Q2527/101
Inventor 林挺马闯闯于祥春黄慧冯晓燕
Owner 艾普拜生物科技(苏州)有限公司