Preparation method of long-term anti-pollution electrochemical biosensor based on human serum albumin molecularly imprinted polymer gel

Abstract

The invention belongs to the field of electrochemical biosensors, and particularly relates to a preparation method of a long-term anti-pollution electrochemical biosensor based on human serum albumin molecularly imprinted polymer gel. The method is realized by the following steps: pretreating a glassy carbon electrode, preparing a sodium alginate mixed solution, dropwise adding the mixed solution to the surface of the pretreated glassy carbon electrode, dropwise adding a calcium salt solution, and after the reaction is finished, eluting template protein molecules by adopting a lauryl sodium sulfate solution to obtain the electrochemical biosensor. The protein molecularly imprinted polymer gel prepared by the invention can effectively eliminate non-specific adsorption of other proteins (bacteria) in a real sample on a sensing interface for a long time (25 days) while realizing selective detection of target molecules, so the specificity, the accuracy and the anti-pollution capacity of a sensor are remarkably improved; the method has the advantages of high selective binding capacity, high accuracy, simplicity in operation, high sensitivity, simple preparation process and low cost.

Description

technical field [0001] The invention belongs to the field of electrochemical biosensors, and in particular relates to a preparation method of an electrochemical biosensor based on human serum albumin molecularly imprinted polymer gel for long-term anti-pollution. Background technique [0002] Molecularly imprinted polymers have the ability to mimic natural receptor molecules, and have increasingly become an important class of artificial synthetic materials. At present, the existing molecularly imprinted polymers of human serum albumin are mainly prepared on the basis of other shapes such as spherical or columnar materials. , the molecularly imprinted polymers synthesized by this type of technology are limited in connection with the electrode surface, making it difficult to be directly applied to the field of sensors; thirdly, the molecularly imprinted polymers prepared by this type of technology hardly eliminate or reduce the non-specific adsorption of other proteins The ab...

AI Technical Summary

Problems solved by technology

At present, the existing molecularly imprinted polymers of human serum albumin are mainly prepared on the basis of other shapes such as spherical or columnar materials. , the molecularly imprinted polymers synthesized by this type of technology are limited in connection with the electrode surface, which makes it difficult to be directly applied to the field of sensors; thirdly, the molecularly imprinted polymers prepared by this type of technology hardly eliminate or reduce the non-specific adsorption of other proteins ability, which directly weakens its ability to analyze and detect targets in actual samples

[0003] In the prior art, most biosensors encounter non-specific adsorption of other proteins and adhesion of cells in the detection of actual samples (including blood, serum, sweat, urine, tissue extracts, cell crushing fluid, etc.), which leads to sensor failure. Low selectivity, poor sensitivity, low accuracy, etc.

At present, there is no report on the preparation of long-term anti-pollution materials using human serum albumin as a template molecule

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