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Fluorescence detection method of sulfur ions and application thereof

A technology for fluorescence detection and sulfide ions, which is applied in fluorescence/phosphorescence, measurement devices, preparation of test samples, etc., can solve the problems of poor water solubility of organic fluorescent probes, poor resistance to photobleaching, and influence on signal-to-noise ratio. Low cost, wide applicability and simple operation

Active Publication Date: 2022-03-22
GUANGZHOU UNIVERSITY
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  • Abstract
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Problems solved by technology

[0004] In recent years H 2 Although some progress has been made in the fluorescence detection of S, there are still challenges: (1) Due to the nonspecificity of fluorescent probes, biothiols will interfere with H 2 Determination of S; (2) Traditional organic fluorescent probes usually have poor water solubility and poor photobleaching resistance; (3) For real biological samples, the autofluorescence produced by proteins and peptides will also affect the signal-to-noise ratio

Method used

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  • Fluorescence detection method of sulfur ions and application thereof
  • Fluorescence detection method of sulfur ions and application thereof
  • Fluorescence detection method of sulfur ions and application thereof

Examples

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Embodiment 1

[0035] In this embodiment, the excitation wavelength selection of the detection system is optimized, and the specific process is as follows:

[0036] Add 100 μL of carbon quantum dot nanomaterial solution (100 μg / mL) into a buffer solution (10 mM) with a pH of 7.4, the total volume of the solution is 1 mL, and mix well. The excitation wavelength is set to 340nm, and the fluorescence intensity of the measurement system at 490nm and 562nm is obtained, and the optimal excitation and emission wavelengths of the carbon quantum dot nanomaterial are obtained. The results are as follows figure 1 .

[0037] from figure 1 It can be seen that carbon quantum dots are a ratiometric fluorescent probe, and when the excitation wavelength is set to 340nm, there are relatively obvious absorption peaks at 490nm and 562nm.

Embodiment 2

[0039] In this implementation, the pH value of the detection system is optimized, and the specific process is as follows:

[0040] Add 100 μL of carbon quantum dot nanomaterial solution (100 μg / mL) to a buffer solution (10 mM) containing 70 μM sulfur ions at different pH values ​​(2-10), the total volume of the solution is 1 mL, and mix well. The excitation wavelength is set to 340nm, and the fluorescence intensity of the system is measured at 490nm and 562nm, the results are as follows figure 2 .

[0041] from figure 2It can be seen that in this system, when the pH is 4-10, the ratio of fluorescence intensity at 562nm / 490nm is obvious, which can effectively reflect the content of sulfur ions in the system; if the pH is too low, the ratio of fluorescence intensity at 562nm / 490nm does not change significantly , resulting in insufficient sensitivity of the detection results; too high a pH does not conform to the real sample detection environment, and has no practical detecti...

Embodiment 3

[0043] The present embodiment has drawn the standard working curve in the detection method, and concrete process is:

[0044] Take 800 μL of sulfur ion solutions with different concentrations (0-70 μM) and mix them with 100 μL carbon quantum dot nanomaterial solution (100 μg / mL) and 100 μL buffer solution (pH7.4, 10 mM); the excitation wavelength is set to 340 nm, and the system is There is an emission peak at 490nm and 562nm, measure the fluorescence intensity of the system at 490nm and 562nm, such as image 3 shown. F in different concentrations of sulfide ion solution 562nm / F 490nm Fluorescence intensity ratio, with the concentration as the abscissa, F 562nm / F 490nm Fluorescence intensity ratio is the ordinate to obtain the standard working curve of sulfide ion detection, the result is as follows Figure 4 shown.

[0045] from image 3 It can be seen that when the concentration of sulfide ion solution is 0-70 μM, the fluorescence intensity at 490nm remains basicall...

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Abstract

The invention provides a fluorescence detection method of sulfur ions and application thereof, and the detection method constructs a sensing system for detecting the sulfur ions by using a fluorescent carbon quantum dot nano material as a fluorescent probe to be applied to sensing detection of the sulfur ions and using a fluorescence intensity ratio of 490nm to 562nm as a fluorescence response signal of the carbon quantum dot nano material to the sulfur ions. The detection method can realize selective detection of sulfur ions, and is not easily influenced by environmental fluctuation factors.

Description

technical field [0001] The invention belongs to the technical field of substance detection, and in particular relates to a fluorescence detection method of sulfur ions and an application thereof. Background technique [0002] Hydrogen sulfide is a colorless, toxic gas that is primarily produced by the decomposition of organic matter or as a by-product of various industries. h 2 S smells like rotten eggs at low concentrations and is odorless at high concentrations. Studies have shown that H 2 S is the third important gas transporter after NO and CO, and is involved in various physiological processes, including relaxation of vascular smooth muscle, mediation of neurotransmission, inhibition of insulin signaling, and regulation of inflammation, among others. If H in the air 2 If the concentration of S exceeds a certain limit, it will lead to rapid paralysis of the respiratory system, loss of thinking ability and balance ability, and even sudden death. [0003] There are ma...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/38
CPCG01N21/643G01N1/38
Inventor 荣铭聪王丹茹包宇牛利黄翊刘汝锋
Owner GUANGZHOU UNIVERSITY
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